The Library
1530 papers on microsampling and monitoring, each with a finding paraphrased to our standard and labelled in one vocabulary. Every entry links to a legitimate copy; nothing is copied from an abstract.
30 papers labelled “dna-genotek-omnigene”, newest first.
2026
Saliva samples collected using the OMNIgene ORAL device and oral wash samples remain stable for microbiome diversity and relative abundance analyses for up to five years when stored at -80°C. This supports the use of these self-collection methods for long-term prospective biobanking and decentralised clinical studies.
Long-term stability at -80°C of oral wash and saliva samples for microbiome analyses — Slack et al., Microbiology spectrum (paywalled)
- stabilised
- dna-genotek-omnigene
- self-collection
- microbiome
- liquid
- validation
- saliva
2026
A study limited by sample size found OMNIgene GUT tubes preserved Cryptosporidium DNA better than DNA Shield and FTA cards after year-long ambient storage. qPCR detected DNA in 23/24 OMNIgene, 21/24 DNA Shield, and 17/20 FTA samples, while metagenomics detected it in 13/24, 9/24, and 0/24 samples respectively, guiding decentralised sampling choices.
Detection of <i>Cryptosporidium hominis</i> by clinical metagenomics in stool samples from an outbreak of diarrhoea among British military personnel in Kenya — Halford et al., BMJ military health (paywalled)
- stabilised
- dna-genotek-omnigene
- serology
- microbiome
- stool
2025
In a field cohort of 60 adults with diarrhoea, OMNIgene 200 and DNA/RNA shield maintained high sensitivity and concordance with fresh samples for most pathogens, while FTA cards showed low sensitivity for STEC and poor specificity for Campylobacter. This supports the use of stabilised stool media for decentralised PCR testing after prolonged ambient transport.
Prospective evaluation of different faecal preservation media for travellers' diarrhoea diagnostic application with multiplex PCR BioFire FilmArray in resource-limited settings — Toriro et al., Journal of medical microbiology
- stabilised
- dna-genotek-omnigene
- serology
- stool
2025
Taxonomic and diversity profiles differed between unstabilised swabs and stabilised OmniGene kits, with transport time disproportionately affecting swab samples; the collection method had a greater impact on taxa and diversity than transport time, highlighting the need for standardised stool collection in decentralised microbiome studies.
Stabilized and unstabilized sampling methods result in differential fecal 16S rRNA microbial sequencing results — Stamper et al., PloS one
- stabilised
- dna-genotek-omnigene
- self-collection
- microbiome
- stool
2025
The study found significant gut microbiota alterations in systemic lupus erythematosus patients, with different beta diversity, p=0.001, and shifts in phyla abundance compared to controls. Specific microbial profiles were associated with clinical subgroups, though the authors note the clinical relevance of species-level alterations requires further validation.
Association of Gut Dysbiosis with Disease Phenotype and Treatment in Systemic Lupus Erythematosus — Medina-Martínez et al., Medical sciences
- stool
- stabilised
- dna-genotek-omnigene
- microbiome
2025
This study compared OMNIgene Gut tubes and FTA cards for stool collection in a deployed setting, finding that OMNIgene yielded higher nucleic acid concentrations while both methods detected the majority of microbial genera. The authors conclude that distinct microbial abundance profiles between the two methods necessitate standardised protocols for field research.
Field expedient stool collection methods for gut microbiome analysis in deployed military environments — Kok et al., mSphere
- stabilised
- dna-genotek-omnigene
- dried
- microbiome
- stool
- validation
2024
This study compared two preservatives for stool samples and found that OMNIgene GUT OMR-200 produced less variation in metagenomic taxonomic data across different storage temperatures, supporting its use in decentralised field studies. The authors recommend absolute quantification to address bias in microbial measurements.
Quantifying bias introduced by sample collection in relative and absolute microbiome measurements — Maghini et al., Nature biotechnology (paywalled)
- stool
- stabilised
- dna-genotek-omnigene
- microbiome
2024
In children, stool preserved in OMNIgene-GUT kept its microbial community structure better than unpreserved stool, with alpha and beta diversity similar to direct freezing, which supports the kit for decentralised stool collection from children.
Omnigene-Gut<sup>tm</sup> ensures fecal microbiome stability in the pediatric population — Hoogendijk et al., AMB Express
- dct
- stabilised
- dna-genotek-omnigene
- microbiome
- pediatric
- stool
- validation
2024
A study found that stool stabilised in 95% ethanol or OMNImet•GUT and OMNIgene•GUT kits maintained metabolome and microbiome profiles comparable to flash freezing for up to seven days at room temperature. Non-stabilised samples showed temperature-dependent changes in bile and short-chain fatty acids, supporting decentralised, patient-centric ambient collection.
Comparative Metabolomics and Microbiome Analysis of Ethanol versus OMNImet/gene•GUT Fecal Stabilization — Isokääntä et al., Analytical chemistry
- stool
- stabilised
- dna-genotek-omnigene
- microbiome
- metabolome
2023
Processing stool samples with OMNIgene SPUTUM significantly improved the diagnostic yield of Mycobacterium tuberculosis using the Xpert MTB/RIF Ultra assay compared to standard methods, offering a viable alternative for patients who cannot produce sputum.
Diagnostic accuracy of Xpert MTB/RIF Ultra and culture assays to detect Mycobacterium Tuberculosis using OMNIgene-sputum processed stool among adult TB presumptive patients in Uganda — Sessolo et al., PloS one
- stool
- stabilised
- dna-genotek-omnigene
- validation
2023
Stool samples collected in DNA/RNA Shield tubes showed better preservation of microbiome taxonomic composition and functional stability over 18 months than OMNIgene-Gut tubes. This demonstrates that ambient stabilisation enables reliable decentralised microbiome collection and long-term storage before analysis.
Long-term taxonomic and functional stability of the gut microbiome from human fecal samples — Kim et al., Scientific reports
- stool
- stabilised
- dna-genotek-omnigene
- validation
- microbiome
2022
TB-MBLA testing of OMNIgene-stabilised stool detected Mycobacterium tuberculosis with 80% sensitivity and 79% specificity against sputum culture in 100 adults, offering a viable alternative for patients unable to produce sputum. The RNA-based assay quantified bacterial load, showing higher burdens in HIV-co-infected individuals, while stool cultures suffered 21-26% contamination rates.
High Mycobacterium tuberculosis Bacillary Loads Detected by Tuberculosis Molecular Bacterial Load Assay in Patient Stool: a Potential Alternative for Nonsputum Diagnosis and Treatment Response Monitoring of Tuberculosis — Musisi et al., Microbiology spectrum
- stool
- stabilised
- dna-genotek-omnigene
- validation
- serology
2021
An ambient-temperature DNA Genotek device recovered 94.5% of the metabolites seen in flash-frozen aliquots with strong agreement: room-temperature stabilisation can stand in for immediate freezing.
An ambient-temperature stabilisation device performs comparably to flash-frozen collection for stool metabolomics in infants — Ramamoorthy et al., BMC Microbiology
- stool
- stabilised
- dna-genotek-omnigene
- metabolome
- validation
- pediatric
2021
OMNIgene·GUT stabilised stool gave detectable total bile acid concentrations but differed significantly from snap frozen samples; however, relative concentrations of cholanic, chenodeoxycholic, deoxycholic and lithocholic acids correlated well with a 30 per cent acceptability bias, supporting its use for decentralised bile acid profiling.
Fitness for purpose of stabilized stool samples for bile acid metabolite analyses — Neuberger-Castillo et al., Scientific reports
- stool
- stabilised
- dna-genotek-omnigene
- validation
- metabolome
2021
DNA extraction methods explained 5.7% of microbiome variability, nearly as much as interindividual differences (7.4%), while collection methods had minimal impact. The choice of kit significantly skewed recovery of Gram-positive bacteria and enterotype distribution, underscoring the need for standardisation in decentralised stool sampling for clinical research.
Quantifying technical confounders in microbiome studies — Bartolomaeus et al., Cardiovascular research (paywalled)
- stool
- stabilised
- dna-genotek-omnigene
- validation
- microbiome
2021
Comparison of six stool collection methods in healthy volunteers found OMNIgene Gut, FOBT cards, RNAlater and Microlution were reliable for metagenomics, whereas 95% ethanol best preserved metabolite profiles; the authors recommend using separate collection methods for different analytical aims in large population studies.
Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics — Guan et al., mSphere
- stool
- stabilised
- dna-genotek-omnigene
- validation
- microbiome
- metabolome
2021
The paper identified that the Zymo DNA/RNA shield preservative paired with the QIAamp Viral RNA Mini Kit extraction yielded more detectable SARS-CoV-2 RNA from stool than the OMNIgene-GUT kit or storage without preservative. This supports decentralised diagnostics by establishing a validated method for preserving and analysing self-collected stool samples, enabling reliable detection of viral shedding for epidemiology and patient management.
Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA — Natarajan et al., Nature communications
- stool
- stabilised
- dna-genotek-omnigene
- validation
- serology
2021
Shotgun metagenomics showed the Copan FLOQSwab in an active drying tube had the best technical and compositional reproducibility among five room temperature methods, outperforming RNALater and OMNIgene-GUT, while LifeGuard and a dry BBL swab allowed unpredictable Escherichia outgrowth; in a further 239-sample evaluation the FLOQSwab-ADT maintained performance across -20 °C, room temperature and 50 °C for four weeks, making it an excellent alternative to existing room temperature stabilisation for stool microbiome diagnostics.
Critical evaluation of faecal microbiome preservation using metagenomic analysis — Pribyl et al., ISME communications
- stool
- stabilised
- dna-genotek-omnigene
- validation
- microbiome
2020
Microbiome profiles remained stable in OMNIgene GUT for 21 days at room temperature and metabolite abundance relationships were preserved, though absolute abundances varied slightly. This supports using a single stool collection procedure with OMNIgene GUT to obtain both microbiome and metabolome data for decentralised diagnostics.
Changes in microbiome and metabolomic profiles of fecal samples stored with stabilizing solution at room temperature: a pilot study — Lim et al., Scientific reports
- stool
- stabilised
- dna-genotek-omnigene
- microbiome
- metabolome
2020
Stock solutions including OMNIgene-Gut maintained stable gut microbial profiles in stool for up to two months at room temperature, supporting their use over freezing for decentralised gut microbiome analysis.
Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperature — Park et al., Journal of microbiology (paywalled)
- stool
- stabilised
- dna-genotek-omnigene
- microbiome
2020
The authors validated an automated DNA extraction method for stool microbiome analysis and found that seven stabilising solutions, including OMNIgene•GUT, RNAlater and AquaStool, preserved microbiome composition as well as snap-freezing. OMNIgene•GUT maintained sample integrity for two years at -80°C. This supports decentralised stool collection for microbiome studies by enabling ambient stabilisation and delayed laboratory processing.
Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis — Neuberger-Castillo et al., Biopreservation and biobanking (paywalled)
- stool
- stabilised
- dna-genotek-omnigene
- validation
- microbiome
2019
Stool stabilised in OMNIgene·GUT then frozen produced 16S and shotgun profiles statistically indistinguishable from fresh-frozen aliquots, supporting stabilised collection where freezing is impractical.
Gut microbiome comparability of fresh-frozen versus stabilised-frozen samples by 16S and shotgun metagenomics — Ilett et al., Scientific Reports
- stool
- stabilised
- dna-genotek-omnigene
- microbiome
- metagenomics
- validation
2019
This study compared four stool collection methods for infant gut microbiome analysis via post and found that the OMNIgene•GUT kit showed the closest agreement with the frozen standard compared to swabs or plain tubes. The findings suggest that stabilised collection offers a viable decentralised solution for parents to sample infant stool at home with minimal DNA degradation.
Gut microbiome analysis by post: Evaluation of the optimal method to collect stool samples from infants within a national cohort study — Williams et al., PloS one
- stool
- stabilised
- dna-genotek-omnigene
- self-collection
- validation
- pediatric
- microbiome
2018
FTA cards and OMNIgene GUT demonstrated strong concordance with immediate freezing for gut microbiome diversity and short-chain fatty acid measurements, while ethanol preserved the most metabolites overall. These stabilised collection methods enable reliable, decentralised stool sampling for large-scale microbiome and metabolomics studies.
Comparison of Fecal Collection Methods for Microbiome and Metabolomics Studies — Wang et al., Frontiers in cellular and infection microbiology
- stool
- stabilised
- dna-genotek-omnigene
- validation
- microbiome
- metabolome
2018
The OMNIgene•GUT kit proved reliable for stool microsampling and ambient storage, with samples stored at room temperature for seven days showing comparable bacterial DNA quantity and diversity to fresh samples; transient phylum-level differences were seen at 24 hours but not at seven days. This supports decentralised, patient-centric microbiome research by enabling home self-collection and postal transport, while emphasising that DNA extraction method has greater impact on microbiome profiles than storage conditions.
Reliability of a participant-friendly fecal collection method for microbiome analyses: a step towards large sample size investigation — Szopinska et al., BMC microbiology
- stool
- stabilised
- dna-genotek-omnigene
- self-collection
- validation
- microbiome
2017
Gammaproteobacteria proliferate in stool samples shipped at room temperature, distorting microbiome profiles. A computational correction method that removes sequences from these blooming taxa enables reliable microbiome analysis from self-collected samples sent without cold chain, producing results comparable to frozen specimens.
Correcting for Microbial Blooms in Fecal Samples during Room-Temperature Shipping — Amir et al., mSystems
- stool
- stabilised
- dna-genotek-omnigene
- self-collection
- validation
- microbiome
2017
In a community-based cohort of elderly men, remote stool self-collection using the OMNIgene·GUT kit achieved high participation and sample adequacy, with mailed samples yielding high-quality DNA for microbiome profiling; this supports decentralised, patient-centric stool sampling for large-scale research.
Successful collection of stool samples for microbiome analyses from a large community-based population of elderly men — Abrahamson et al., Contemporary clinical trials communications
- stool
- stabilised
- dna-genotek-omnigene
- self-collection
- acceptability
- microbiome
2016
Testing 15 individuals and over 1,200 samples, this study found that 95% ethanol, FTA cards, and the OMNIgene Gut kit maintain stool microbiome stability at ambient temperatures for eight weeks. This enables robust decentralised sampling, though sponsors must use a single method to prevent batch effects and avoid 70% ethanol.
Preservation Methods Differ in Fecal Microbiome Stability, Affecting Suitability for Field Studies — Song et al., mSystems
- stool
- stabilised
- dna-genotek-omnigene
- microbiome
2016
The study found that ambient temperature collection and stabilisation of stool using the DNA Genotek OMNIgene·Gut device yielded the same data reproducibility as freezing and higher recovery of nucleic acids, enabling standardised global collection and analysis for microbiome studies.
A robust ambient temperature collection and stabilization strategy: Enabling worldwide functional studies of the human microbiome — Anderson et al., Scientific reports
- stabilised
- dna-genotek-omnigene
- microbiome
- stool
- validation
2016
OMNIgene GUT vials improved stool DNA quality by reducing shearing, but storage for one week altered microbiota composition in pooled infant and elderly data, with infant samples more affected. For decentralised collection, extraction within the first week and a consistent storage regime are advisable.
Effect of room temperature transport vials on DNA quality and phylogenetic composition of faecal microbiota of elderly adults and infants — Hill et al., Microbiome
- stool
- stabilised
- dna-genotek-omnigene
- validation
- pediatric
- microbiome
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