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OpenSampling

The Library

1530 papers on microsampling and monitoring, each with a finding paraphrased to our standard and labelled in one vocabulary. Every entry links to a legitimate copy; nothing is copied from an abstract.

52 papers labelled “stabilised”, newest first.

  1. 2026

    Participants preferred the Omnigene and Zymo oral swabs for comfort and ease of use, whilst the Omnigene device yielded the highest median bacterial DNA concentration compared to the other devices. The results indicate that the choice of self-collection device impacts both patient acceptability and DNA yield for oral microbiome analysis.

    Comparison of self-collected oral swabs for supragingival microbiome characterization using 16S rRNA gene amplicon sequencingRooney et al., Journal of microbiological methods (paywalled)

    • acceptability
    • stabilised
    • self-collection
    • microbiome
    • saliva
  2. 2026

    Saliva samples collected using the OMNIgene ORAL device and oral wash samples remain stable for microbiome diversity and relative abundance analyses for up to five years when stored at -80°C. This supports the use of these self-collection methods for long-term prospective biobanking and decentralised clinical studies.

    Long-term stability at -80°C of oral wash and saliva samples for microbiome analysesSlack et al., Microbiology spectrum (paywalled)

    • stabilised
    • dna-genotek-omnigene
    • self-collection
    • microbiome
    • liquid
    • validation
    • saliva
  3. 2026

    A study limited by sample size found OMNIgene GUT tubes preserved Cryptosporidium DNA better than DNA Shield and FTA cards after year-long ambient storage. qPCR detected DNA in 23/24 OMNIgene, 21/24 DNA Shield, and 17/20 FTA samples, while metagenomics detected it in 13/24, 9/24, and 0/24 samples respectively, guiding decentralised sampling choices.

    Detection of <i>Cryptosporidium hominis</i> by clinical metagenomics in stool samples from an outbreak of diarrhoea among British military personnel in KenyaHalford et al., BMJ military health (paywalled)

    • stabilised
    • dna-genotek-omnigene
    • serology
    • microbiome
    • stool
  4. 2026

    The homeRNA capillary blood collection and stabilization platform successfully captured lipopolysaccharide-induced inflammatory gene expression profiles. These transcriptomic responses were comparable to those obtained from traditional venous blood samples stabilized with RNAlater or PAXgene, demonstrating the platform's suitability for remote transcriptomic monitoring.

    From Home to Transcriptome: Comparing the Transcriptomic Profile of Induced Immune Response via Lipopolysaccharide Stimulation in homeRNA and Venous BloodBrown et al., Analytical chemistry (paywalled)

    • venous-agreement
    • stabilised
    • blood
    • capillary
    • liquid
    • validation
    • biomarkers
  5. 2026

    The True Dose TD-EPI kit demonstrated strong analytical agreement with venous blood for epirubicin monitoring, and the capillary samples remained stable for 72 hours at ambient temperature.

    Method-Comparison Validation of a Novel Capillary Blood Collection Kit, True Dose<sup>®</sup> TD-EPI, for Therapeutic Drug Monitoring of EpirubicinDe Chiara et al., Pharmaceuticals

    • venous-agreement
    • stabilised
    • blood
    • tdm
    • oncology
    • capillary
    • liquid
    • validation
  6. 2025

    In a field cohort of 60 adults with diarrhoea, OMNIgene 200 and DNA/RNA shield maintained high sensitivity and concordance with fresh samples for most pathogens, while FTA cards showed low sensitivity for STEC and poor specificity for Campylobacter. This supports the use of stabilised stool media for decentralised PCR testing after prolonged ambient transport.

    Prospective evaluation of different faecal preservation media for travellers' diarrhoea diagnostic application with multiplex PCR BioFire FilmArray in resource-limited settingsToriro et al., Journal of medical microbiology

    • stabilised
    • dna-genotek-omnigene
    • serology
    • stool
  7. 2025

    Taxonomic and diversity profiles differed between unstabilised swabs and stabilised OmniGene kits, with transport time disproportionately affecting swab samples; the collection method had a greater impact on taxa and diversity than transport time, highlighting the need for standardised stool collection in decentralised microbiome studies.

    Stabilized and unstabilized sampling methods result in differential fecal 16S rRNA microbial sequencing resultsStamper et al., PloS one

    • stabilised
    • dna-genotek-omnigene
    • self-collection
    • microbiome
    • stool
  8. 2025

    The study found significant gut microbiota alterations in systemic lupus erythematosus patients, with different beta diversity, p=0.001, and shifts in phyla abundance compared to controls. Specific microbial profiles were associated with clinical subgroups, though the authors note the clinical relevance of species-level alterations requires further validation.

    Association of Gut Dysbiosis with Disease Phenotype and Treatment in Systemic Lupus ErythematosusMedina-Martínez et al., Medical sciences

    • stool
    • stabilised
    • dna-genotek-omnigene
    • microbiome
  9. 2025

    This study compared OMNIgene Gut tubes and FTA cards for stool collection in a deployed setting, finding that OMNIgene yielded higher nucleic acid concentrations while both methods detected the majority of microbial genera. The authors conclude that distinct microbial abundance profiles between the two methods necessitate standardised protocols for field research.

    Field expedient stool collection methods for gut microbiome analysis in deployed military environmentsKok et al., mSphere

    • stabilised
    • dna-genotek-omnigene
    • dried
    • microbiome
    • stool
    • validation
  10. 2024

    This study compared two preservatives for stool samples and found that OMNIgene GUT OMR-200 produced less variation in metagenomic taxonomic data across different storage temperatures, supporting its use in decentralised field studies. The authors recommend absolute quantification to address bias in microbial measurements.

    Quantifying bias introduced by sample collection in relative and absolute microbiome measurementsMaghini et al., Nature biotechnology (paywalled)

    • stool
    • stabilised
    • dna-genotek-omnigene
    • microbiome
  11. 2024

    In children, stool preserved in OMNIgene-GUT kept its microbial community structure better than unpreserved stool, with alpha and beta diversity similar to direct freezing, which supports the kit for decentralised stool collection from children.

    Omnigene-Gut<sup>tm</sup> ensures fecal microbiome stability in the pediatric populationHoogendijk et al., AMB Express

    • dct
    • stabilised
    • dna-genotek-omnigene
    • microbiome
    • pediatric
    • stool
    • validation
  12. 2024

    A study found that stool stabilised in 95% ethanol or OMNImet•GUT and OMNIgene•GUT kits maintained metabolome and microbiome profiles comparable to flash freezing for up to seven days at room temperature. Non-stabilised samples showed temperature-dependent changes in bile and short-chain fatty acids, supporting decentralised, patient-centric ambient collection.

    Comparative Metabolomics and Microbiome Analysis of Ethanol versus OMNImet/gene•GUT Fecal StabilizationIsokääntä et al., Analytical chemistry

    • stool
    • stabilised
    • dna-genotek-omnigene
    • microbiome
    • metabolome
  13. 2023

    Across 18 saliva samples, mouthwash collection gave higher alpha diversity than passive drooling on both Illumina and Nanopore platforms, and three preservation methods performed alike; mouthwash and simple collection are convenient decentralised options, provided a study keeps one collection method for all participants to avoid confounding.

    Factors influencing oral microbiome analysis: from saliva sampling to sequencing platformsBang et al., Scientific Reports

    • stabilised
    • microbiome
    • liquid
    • validation
    • saliva
  14. 2023

    The study compared two stool collection devices and found that GutAlive maintained bacterial viability and DNA integrity over time, preserving the original microbiome composition and diversity, which supports its use for decentralised self-collection in microbiome diagnostics.

    GutAlive<sup>®</sup> enables DNA-based microbiome analysis without disrupting the original composition and diversityMontero et al., Frontiers in microbiology

    • stabilised
    • self-collection
    • microbiome
    • stool
  15. 2023

    Stool from two volunteers showed different microbiome profiles between the outer cortex and the inner core, and homogenising then stabilising at 4°C for 24 hours preserved diversity better than fresh or frozen processing; a possible route to home stool collection, though only two people were sampled.

    Accelerating Gut Microbiome Research with Robust Sample CollectionZreloff et al., Research & reviews. Journal of microbiology and biotechnology

    • stool
    • stabilised
    • microbiome
  16. 2023

    Processing stool samples with OMNIgene SPUTUM significantly improved the diagnostic yield of Mycobacterium tuberculosis using the Xpert MTB/RIF Ultra assay compared to standard methods, offering a viable alternative for patients who cannot produce sputum.

    Diagnostic accuracy of Xpert MTB/RIF Ultra and culture assays to detect Mycobacterium Tuberculosis using OMNIgene-sputum processed stool among adult TB presumptive patients in UgandaSessolo et al., PloS one

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
  17. 2023

    Fecal and oral microbiome samples showed high stability over two years at -80°C, with intraclass correlation coefficients of 0.70–0.99 for stool and above 0.74 for saliva across most collection methods. Stool collected without additive and saliva collected with Scope mouthwash showed lower stability for some measures, demonstrating that collection method choice affects reliability of stored samples for microbiome analysis in decentralised diagnostics.

    Stability of the Fecal and Oral Microbiome over 2 Years at -80°C for Multiple Collection MethodsZouiouich et al., Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology (paywalled)

    • saliva
    • stool
    • stabilised
    • validation
    • microbiome
  18. 2023

    Stool samples collected in DNA/RNA Shield tubes showed better preservation of microbiome taxonomic composition and functional stability over 18 months than OMNIgene-Gut tubes. This demonstrates that ambient stabilisation enables reliable decentralised microbiome collection and long-term storage before analysis.

    Long-term taxonomic and functional stability of the gut microbiome from human fecal samplesKim et al., Scientific reports

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • microbiome
  19. 2022

    A dissolvable wipe with DESS solution captured stool for metagenomics with high species-level agreement to frozen storage, R2 0.96, preserved Shannon diversity and species richness, and performed comparably to a commercial preservation kit, R2 0.98, enabling stable room temperature collection and transport for decentralised sampling.

    A Wipe-Based Stool Collection and Preservation Kit for Microbiome Community ProfilingHua et al., Frontiers in immunology

    • stabilised
    • self-collection
    • microbiome
    • stool
    • validation
  20. 2022

    TB-MBLA testing of OMNIgene-stabilised stool detected Mycobacterium tuberculosis with 80% sensitivity and 79% specificity against sputum culture in 100 adults, offering a viable alternative for patients unable to produce sputum. The RNA-based assay quantified bacterial load, showing higher burdens in HIV-co-infected individuals, while stool cultures suffered 21-26% contamination rates.

    High Mycobacterium tuberculosis Bacillary Loads Detected by Tuberculosis Molecular Bacterial Load Assay in Patient Stool: a Potential Alternative for Nonsputum Diagnosis and Treatment Response Monitoring of TuberculosisMusisi et al., Microbiology spectrum

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • serology
  21. 2021

    An ambient-temperature DNA Genotek device recovered 94.5% of the metabolites seen in flash-frozen aliquots with strong agreement: room-temperature stabilisation can stand in for immediate freezing.

    An ambient-temperature stabilisation device performs comparably to flash-frozen collection for stool metabolomics in infantsRamamoorthy et al., BMC Microbiology

    • stool
    • stabilised
    • dna-genotek-omnigene
    • metabolome
    • validation
    • pediatric
  22. 2021

    A study found that fecal samples on FOBT cards and RNAlater, and oral samples in Scope mouthwash, remained stable for four days at room temperature, with fecal comparability ICCs ranging from 0.63 to 0.93. These methods support decentralised sampling, though consistent method choice is required as each may introduce modest differences.

    Comparison of fecal and oral collection methods for studies of the human microbiota in two Iranian cohortsWu et al., BMC microbiology

    • saliva
    • stool
    • stabilised
    • microbiome
  23. 2021

    OMNIgene·GUT stabilised stool gave detectable total bile acid concentrations but differed significantly from snap frozen samples; however, relative concentrations of cholanic, chenodeoxycholic, deoxycholic and lithocholic acids correlated well with a 30 per cent acceptability bias, supporting its use for decentralised bile acid profiling.

    Fitness for purpose of stabilized stool samples for bile acid metabolite analysesNeuberger-Castillo et al., Scientific reports

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • metabolome
  24. 2021

    DNA extraction methods explained 5.7% of microbiome variability, nearly as much as interindividual differences (7.4%), while collection methods had minimal impact. The choice of kit significantly skewed recovery of Gram-positive bacteria and enterotype distribution, underscoring the need for standardisation in decentralised stool sampling for clinical research.

    Quantifying technical confounders in microbiome studiesBartolomaeus et al., Cardiovascular research (paywalled)

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • microbiome
  25. 2021

    Comparison of six stool collection methods in healthy volunteers found OMNIgene Gut, FOBT cards, RNAlater and Microlution were reliable for metagenomics, whereas 95% ethanol best preserved metabolite profiles; the authors recommend using separate collection methods for different analytical aims in large population studies.

    Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted MetabolomicsGuan et al., mSphere

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • microbiome
    • metabolome
  26. 2021

    Instability of salivary biomarkers after collection and the absence of standardised handling methods are the principal barriers to home-based saliva diagnostics. The review collates preservation strategies for proteins, hormones, and nucleic acids that are essential for reliable decentralised testing.

    Stabilization of Salivary Biomarkersd'Amone et al., ACS biomaterials science & engineering (paywalled)

    • saliva
    • stabilised
    • self-collection
    • validation
    • biomarkers
  27. 2021

    The paper identified that the Zymo DNA/RNA shield preservative paired with the QIAamp Viral RNA Mini Kit extraction yielded more detectable SARS-CoV-2 RNA from stool than the OMNIgene-GUT kit or storage without preservative. This supports decentralised diagnostics by establishing a validated method for preserving and analysing self-collected stool samples, enabling reliable detection of viral shedding for epidemiology and patient management.

    Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNANatarajan et al., Nature communications

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • serology
  28. 2021

    Shotgun metagenomics showed the Copan FLOQSwab in an active drying tube had the best technical and compositional reproducibility among five room temperature methods, outperforming RNALater and OMNIgene-GUT, while LifeGuard and a dry BBL swab allowed unpredictable Escherichia outgrowth; in a further 239-sample evaluation the FLOQSwab-ADT maintained performance across -20 °C, room temperature and 50 °C for four weeks, making it an excellent alternative to existing room temperature stabilisation for stool microbiome diagnostics.

    Critical evaluation of faecal microbiome preservation using metagenomic analysisPribyl et al., ISME communications

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • microbiome
  29. 2020

    The study found that the OMNIgene ORAL kit produced different microbiome profiles compared to some alternative oral collection methods, particularly Saccomanno's fixative. This matters for decentralised sampling as it shows the choice of a standardised, stabilised collection method is critical for reliable results in remote studies of the oral microbiome.

    Comparison of Oral Microbiota Collected Using Multiple Methods and Recommendations for New Epidemiologic StudiesYano et al., mSystems

    • stabilised
    • microbiome
    • validation
    • saliva
  30. 2020

    Microbiome profiles remained stable in OMNIgene GUT for 21 days at room temperature and metabolite abundance relationships were preserved, though absolute abundances varied slightly. This supports using a single stool collection procedure with OMNIgene GUT to obtain both microbiome and metabolome data for decentralised diagnostics.

    Changes in microbiome and metabolomic profiles of fecal samples stored with stabilizing solution at room temperature: a pilot studyLim et al., Scientific reports

    • stool
    • stabilised
    • dna-genotek-omnigene
    • microbiome
    • metabolome
  31. 2020

    Stock solutions including OMNIgene-Gut maintained stable gut microbial profiles in stool for up to two months at room temperature, supporting their use over freezing for decentralised gut microbiome analysis.

    Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperaturePark et al., Journal of microbiology (paywalled)

    • stool
    • stabilised
    • dna-genotek-omnigene
    • microbiome
  32. 2020

    The authors validated an automated DNA extraction method for stool microbiome analysis and found that seven stabilising solutions, including OMNIgene•GUT, RNAlater and AquaStool, preserved microbiome composition as well as snap-freezing. OMNIgene•GUT maintained sample integrity for two years at -80°C. This supports decentralised stool collection for microbiome studies by enabling ambient stabilisation and delayed laboratory processing.

    Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome AnalysisNeuberger-Castillo et al., Biopreservation and biobanking (paywalled)

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • microbiome
  33. 2019

    Stool stabilised in OMNIgene·GUT then frozen produced 16S and shotgun profiles statistically indistinguishable from fresh-frozen aliquots, supporting stabilised collection where freezing is impractical.

    Gut microbiome comparability of fresh-frozen versus stabilised-frozen samples by 16S and shotgun metagenomicsIlett et al., Scientific Reports

    • stool
    • stabilised
    • dna-genotek-omnigene
    • microbiome
    • metagenomics
    • validation
  34. 2019

    This study compared four stool collection methods for infant gut microbiome analysis via post and found that the OMNIgene•GUT kit showed the closest agreement with the frozen standard compared to swabs or plain tubes. The findings suggest that stabilised collection offers a viable decentralised solution for parents to sample infant stool at home with minimal DNA degradation.

    Gut microbiome analysis by post: Evaluation of the optimal method to collect stool samples from infants within a national cohort studyWilliams et al., PloS one

    • stool
    • stabilised
    • dna-genotek-omnigene
    • self-collection
    • validation
    • pediatric
    • microbiome
  35. 2019

    OMNIgene SPUTUM treated sputum specimens stored and transported at ambient temperature for up to 29 days yielded GeneXpert MTB/RIF results comparable to cold chain samples, with very good inter test agreement (kappa 0.97) and 99% overall agreement, supporting decentralised testing without cold chain.

    Comparative study of OMNIgene®•SPUTUM reagent versus cold-chain for the transportation of sputum samples to GeneXpert®MTB/RIF testing sites in MalawiAlemayehu et al., BMC infectious diseases

    • stabilised
  36. 2019

    Oral rinse samples collected in Scope mouthwash remained stable at ambient temperature for four days, with high intraclass correlation for microbial diversity (Shannon index ICC 0.86), supporting their use in decentralised microbiome studies. However, comparability with OMNIgene ORAL samples was low, suggesting collection methods should not be mixed within a study.

    Comparison of Oral Collection Methods for Studies of MicrobiotaVogtmann et al., Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology (paywalled)

    • saliva
    • stabilised
    • validation
    • microbiome
  37. 2018

    FTA cards and OMNIgene GUT demonstrated strong concordance with immediate freezing for gut microbiome diversity and short-chain fatty acid measurements, while ethanol preserved the most metabolites overall. These stabilised collection methods enable reliable, decentralised stool sampling for large-scale microbiome and metabolomics studies.

    Comparison of Fecal Collection Methods for Microbiome and Metabolomics StudiesWang et al., Frontiers in cellular and infection microbiology

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • microbiome
    • metabolome
  38. 2018

    Oral microbiota alpha diversity remained stable over 10 months of self-collected samples (intraclass correlation 0.74–0.79), though relative abundance of specific taxa varied more. These stability estimates allow proper power calculations for decentralised epidemiologic studies using oral microsampling.

    Temporal Variability of Oral Microbiota over 10 Months and the Implications for Future Epidemiologic StudiesVogtmann et al., Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology (paywalled)

    • saliva
    • stabilised
    • microbiome
  39. 2018

    The OMNIgene•GUT kit proved reliable for stool microsampling and ambient storage, with samples stored at room temperature for seven days showing comparable bacterial DNA quantity and diversity to fresh samples; transient phylum-level differences were seen at 24 hours but not at seven days. This supports decentralised, patient-centric microbiome research by enabling home self-collection and postal transport, while emphasising that DNA extraction method has greater impact on microbiome profiles than storage conditions.

    Reliability of a participant-friendly fecal collection method for microbiome analyses: a step towards large sample size investigationSzopinska et al., BMC microbiology

    • stool
    • stabilised
    • dna-genotek-omnigene
    • self-collection
    • validation
    • microbiome
  40. 2017

    Gammaproteobacteria proliferate in stool samples shipped at room temperature, distorting microbiome profiles. A computational correction method that removes sequences from these blooming taxa enables reliable microbiome analysis from self-collected samples sent without cold chain, producing results comparable to frozen specimens.

    Correcting for Microbial Blooms in Fecal Samples during Room-Temperature ShippingAmir et al., mSystems

    • stool
    • stabilised
    • dna-genotek-omnigene
    • self-collection
    • validation
    • microbiome
  41. 2017

    In a community-based cohort of elderly men, remote stool self-collection using the OMNIgene·GUT kit achieved high participation and sample adequacy, with mailed samples yielding high-quality DNA for microbiome profiling; this supports decentralised, patient-centric stool sampling for large-scale research.

    Successful collection of stool samples for microbiome analyses from a large community-based population of elderly menAbrahamson et al., Contemporary clinical trials communications

    • stool
    • stabilised
    • dna-genotek-omnigene
    • self-collection
    • acceptability
    • microbiome
  42. 2017

    This study found that saliva collected with the OMNIgene ORAL kit and stored at room temperature for up to 13 months allowed for the detection of Plasmodium falciparum DNA with high sensitivity and specificity compared to blood tests, suggesting it is a viable non-invasive alternative for malaria diagnosis.

    Detection of Plasmodium falciparum DNA in saliva samples stored at room temperature: potential for a non-invasive saliva-based diagnostic test for malariaMfuh et al., Malaria journal

    • saliva
    • stabilised
    • validation
    • serology
  43. 2016

    With Oragene DISCOVER self-collection, saliva SNP-array genotypes matched blood at 99.996% concordance; about 6% of samples failed on low yield, so an occasional repeat should be expected.

    Saliva DNA quality and genotyping efficiency in a predominantly elderly populationGudiseva et al., BMC Medical Genomics

    • dna
    • venous-agreement
    • stabilised
    • blood
    • dna-genotek-oragene
    • validation
    • saliva
    • genotyping
  44. 2016

    ORAcollect saliva matched blood at 97.9% overall variant concordance on a hereditary-cancer NGS panel, rising to 100% for protein-coding variants, supporting self-collected saliva for germline testing.

    Validation of saliva as an alternative specimen for detecting hereditary breast-cancer mutations by next-generation sequencingMeghnani et al., International J. Genomics

    • dna
    • venous-agreement
    • stabilised
    • blood
    • dna-genotek-oracollect
    • validation
    • saliva
    • genotyping
  45. 2016

    Testing 15 individuals and over 1,200 samples, this study found that 95% ethanol, FTA cards, and the OMNIgene Gut kit maintain stool microbiome stability at ambient temperatures for eight weeks. This enables robust decentralised sampling, though sponsors must use a single method to prevent batch effects and avoid 70% ethanol.

    Preservation Methods Differ in Fecal Microbiome Stability, Affecting Suitability for Field StudiesSong et al., mSystems

    • stool
    • stabilised
    • dna-genotek-omnigene
    • microbiome
  46. 2016

    The study found that ambient temperature collection and stabilisation of stool using the DNA Genotek OMNIgene·Gut device yielded the same data reproducibility as freezing and higher recovery of nucleic acids, enabling standardised global collection and analysis for microbiome studies.

    A robust ambient temperature collection and stabilization strategy: Enabling worldwide functional studies of the human microbiomeAnderson et al., Scientific reports

    • stabilised
    • dna-genotek-omnigene
    • microbiome
    • stool
    • validation
  47. 2016

    OMNIgene GUT vials improved stool DNA quality by reducing shearing, but storage for one week altered microbiota composition in pooled infant and elderly data, with infant samples more affected. For decentralised collection, extraction within the first week and a consistent storage regime are advisable.

    Effect of room temperature transport vials on DNA quality and phylogenetic composition of faecal microbiota of elderly adults and infantsHill et al., Microbiome

    • stool
    • stabilised
    • dna-genotek-omnigene
    • validation
    • pediatric
    • microbiome
  48. 2016

    Saliva microbiomes stored in OMNIgene medium remained stable for at least one week at room temperature. Dental plaque collected by scaler or CytoSoft brush showed minimal taxonomic differences, while plaque stored in OMNIgene exhibited higher diversity and greater similarity between samples than plaque in liquid transport medium. These results inform ambient stabilisation protocols for decentralised oral microbiome collection.

    Effects of Specimen Collection Methodologies and Storage Conditions on the Short-Term Stability of Oral Microbiome TaxonomyLuo et al., Applied and environmental microbiology (paywalled)

    • saliva
    • stabilised
    • pediatric
    • microbiome
  49. 2015

    The Powermicrobiome Kit yielded the best RNA from stool, and RNA Later preserved mRNA integrity for six days at room temperature, though it introduced taxonomic and functional bias. RNA Protect was unsuitable beyond 24 hours. These findings support decentralised stool collection for metatranscriptomics but highlight the need to account for stabilisation reagent effects.

    Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolationReck et al., BMC genomics

    • stool
    • stabilised
    • validation
    • microbiome
  50. 2015

    The DNA Genotek Oragene RNA assay yielded approximately four times more RNA from neonatal saliva than the Qiagen RNeasy kit and showed stronger correlation with RT-qPCR performance, though it required nearly twice the extraction time; RNA integrity was comparable between the two systems.

    Comparative performance analyses of commercially available products for salivary collection and nucleic acid processing in the newbornMaron & Johnson, Biotechnic & histochemistry : official publication of the Biological Stain Commission (paywalled)

    • saliva
    • stabilised
    • dna-genotek-oragene
    • pediatric
  51. 2015

    The OMNIgene™·DISCOVER kit preserved viral DNA integrity in saliva for up to 14 months at room temperature, outperforming frozen storage which caused significant degradation. Viral loads remained stable for 6-9 months with the kit, supporting its use for decentralised diagnostic testing in resource-constrained settings.

    Detecting DNA viruses in oral fluids: evaluation of collection and storage methodsSpeicher et al., Diagnostic microbiology and infectious disease (paywalled)

    • saliva
    • stabilised
    • validation
    • serology
  52. 2012

    Oragene-stabilised saliva DNA agreed with blood at 98.7% of called markers on a pharmacogenetic array; saliva's lower overall call rate tracked its variable human-DNA content.

    Genotyping Performance between Saliva and Blood-Derived Genomic DNAs on the DMET Array: A ComparisonHu et al., PLoS ONE

    • dna
    • stabilised
    • blood
    • dna-genotek-oragene
    • validation
    • saliva
    • genotyping

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