The Library
1530 papers on microsampling and monitoring, each with a finding paraphrased to our standard and labelled in one vocabulary. Every entry links to a legitimate copy; nothing is copied from an abstract.
28 papers labelled “dna-genotek-oragene”, newest first.
2024
Using the Oragene saliva device, this study identified elevated CYP2C8 allele frequencies in a Saudi paediatric cohort compared to other Asian populations, suggesting potential variability in ibuprofen metabolism and drug response.
Pediatric pharmacogenetics: profiling CYP2C8 polymorphisms at King Abdulaziz University Dental Clinic — Bagher et al., Drug metabolism and personalized therapy (paywalled)
- saliva
- dna-genotek-oragene
- pediatric
- genotyping
2023
Using the Oragene Discovery 500 series for decentralised saliva collection in 524 African American adolescents, this study found that positive neighbourhood conditions relate to fewer behavioural difficulties, despite genetic risk. The authors note that most gene-environment studies of behaviour use European ancestry samples, limiting generalisability to African American youth.
Genetic Risk, Neighborhood Characteristics, and Behavioral Difficulties Among African American Adolescents Living in Very Low-Income Neighborhoods — Sterrett-Hong et al., Research on child and adolescent psychopathology (paywalled)
- dna-genotek-oragene
- pediatric
- saliva
- genotyping
2023
Using patient-centric saliva microsampling with the Oragene kit, the study found a low overall prevalence, 13%, of the OPRM1 118G allele in a Western Saudi population, with a significantly higher frequency in females, 21%, than males, 5%. The authors note the findings are specific to this population and may not be generalisable.
Allele frequency and genotype distribution of the opioid receptor μ-1 (OPRM1) A118G polymorphism in the Western Saudi population — Bagher & Hareeri, Journal of applied biomedicine (paywalled)
- saliva
- dna-genotek-oragene
- genotyping
2023
The study found that combining the Oragene saliva collector with the miRNeasy kit gave the most sensitive detection of seven saliva miRNAs, and that different collector-kit combinations can be selected to suit different study needs.
Obtaining miRNA from Saliva-Comparison of Sampling and Purification Methods — Urbizu et al., International journal of molecular sciences
- saliva
- liquid
- dna-genotek-oragene
- validation
- biomarkers
2023
The study found that the Oragene OG-600 receptacle provided the highest concentration of salivary DNA, including short fragments suitable for analysis, and successfully detected HPV DNA from patient saliva. This matters for decentralised sampling as it supports the use of a standardised, self-collection device for non-invasive cancer biomarker detection.
Isolation of salivary cell-free DNA for cancer detection — Brooks et al., PloS one
- dna-genotek-oragene
- hpv
- liquid-biopsy
- liquid
- saliva
- genotyping
2020
Self-collected saliva samples provided adequate DNA for genotyping in preterm birth research, though questionnaire data on birth details had limited concordance with prospective records, indicating that while DNA sampling is feasible via post, phenotyping requires more reliable data collection methods for genetic studies.
Postal recruitment for genetic studies of preterm birth: A feasibility study — Keag et al., Wellcome open research
- dct
- dna-genotek-oragene
- self-collection
- liquid
- saliva
- genotyping
2020
Testing 130 healthy adults, researchers found that a caffeine breath test can identify the rs4410790 genotype using saliva collected via the Oragene DNA saliva collection kit. However, breath metrics did not differ for rs762551 or rs5751876 genotypes, and all subjects had the CC genotype at rs2472297.
<sup>13</sup>C-caffeine breath test identifies single nucleotide polymorphisms associated with caffeine metabolism — Ishii et al., Drug metabolism and pharmacokinetics (paywalled)
- saliva
- dna-genotek-oragene
- genotyping
2020
Using OraGene kits for saliva collection from 210 Portuguese mothers, this study found that the 5-HTTLPR polymorphism moderates environmental effects on maternal sensitivity. While generalisability may be limited by the Portuguese community sample, the work shows that decentralised saliva sampling is viable for gene-environment interaction studies.
Does 5-HTTLPR moderate the effect of the quality of environmental context on maternal sensitivity? Testing the differential susceptibility hypothesis — Baião et al., Psychiatric genetics (paywalled)
- saliva
- dna-genotek-oragene
- genotyping
2020
Using the Oragene DNA self-collection kit, this study found no significant difference in CNTFR genotype frequencies between weightlifters, 56% CC, 32% CT and 12% TT, and controls, 53% CC, 40% CT and 7% TT. However, the CT+TT genotype was associated with higher relative performance, though the cross-sectional design limits causal inference.
Ciliary Neurotrophic Factor Receptor rs41274853 Polymorphism Is Associated With Weightlifting Performance in Japanese Weightlifters — Homma et al., Journal of strength and conditioning research (paywalled)
- saliva
- dna-genotek-oragene
- self-collection
- genotyping
2020
Using a patient-centric saliva microsampling kit, the study found that genetic variants, VKORC1 and CYP2C9, and smoking explained 39.2% of warfarin dose variability in a Qatari population, supporting decentralised sampling for pharmacogenomic profiling.
The effect of genetic and nongenetic factors on warfarin dose variability in Qatari population — Bader et al., The pharmacogenomics journal (paywalled)
- tdm
- dna-genotek-oragene
- self-collection
- saliva
- genotyping
2020
This proof-of-principle study found that baseline salivary DNA methylation, collected using the Oragene DNA kit, predicted childhood obesity three years later. A child at the 75th percentile of NRF1 methylation had a 48% chance of obesity versus 30% at the 25th percentile, showing the value of salivary microsampling for paediatric screening.
Salivary epigenetic biomarkers as predictors of emerging childhood obesity — Rushing et al., BMC medical genetics
- saliva
- dna-genotek-oragene
- pediatric
- genotyping
2018
Telomere length measurements vary significantly depending on the sample type used, with Oragene saliva showing a moderate correlation to venous buffy coat, whereas Oasis saliva and dried blood spots did not yield comparable or adequate results for this assay.
Evaluating minimally invasive sample collection methods for telomere length measurement — Goldman et al., American journal of human biology : the official journal of the Human Biology Council (paywalled)
- blood
- saliva
- dried
- dbs
- dna-genotek-oragene
- validation
- venous-agreement
- genotyping
2018
Both blood and saliva collection methods produced sufficient DNA for genotyping, with saliva using Oragene kits being less invasive and more acceptable for participants who declined blood draws, supporting its use in decentralised research settings
The MOSAICC study: Assessing feasibility for biological sample collection in epidemiology studies and comparison of DNA yields from saliva and whole blood samples — James et al., Annals of human genetics (paywalled)
- blood
- saliva
- dna-genotek-oragene
- acceptability
- genotyping
2018
A study of 307 youth aged 10 to 14 years found that DNA extracted from saliva collected with Oragene kits was suitable for genotyping the OXTR rs53576 variant. This supports decentralised paediatric genetic testing, although the study was limited by its reliance on a community sample.
Depression in early adolescence: Contributions from relational aggression and variation in the oxytocin receptor gene — Kushner et al., Aggressive behavior (paywalled)
- dna-genotek-oragene
- pediatric
- saliva
- genotyping
2018
Saliva collected with the Oragene RNA kit contained highly variable amounts of RNA that were predominantly bacterial, with minimal human mRNA present. This limits the immediate utility of salivary transcriptome for disease susceptibility testing in decentralised diagnostics and highlights the need for improved human RNA extraction methods.
Feasibility of the salivary transcriptome as a novel biomarker in determining disease susceptibility — Hidayat et al., Journal of periodontal research (paywalled)
- saliva
- dna-genotek-oragene
- biomarkers
2017
Across five extraction protocols, storage time generally reduced DNA quantity but protocol 1 (Oragene) maintained yield; DNA purity was largely unaffected by storage for commercial protocols but declined for non‑commercial methods; only protocol 1 consistently gave unfragmented DNA and protocols 1–4 yielded amplifiable DNA while protocol 5 rarely did. This supports reliable saliva self‑collection and long‑term storage for genotyping in decentralised settings.
Human DNA extraction from whole saliva that was fresh or stored for 3, 6 or 12 months using five different protocols — Garbieri et al., Journal of applied oral science : revista FOB
- saliva
- dna-genotek-oragene
- validation
- genotyping
2017
Saliva self-collection with Oragene tubes enabled genotyping of COMT polymorphisms in children, revealing that the AA genotype buffers the negative effect of authoritarian parenting on long-term executive dysfunction after early childhood traumatic brain injury. This demonstrates how decentralised genetic sampling can support longitudinal neurodevelopmental studies in paediatric populations.
Catechol-O-Methyltransferase Genotypes and Parenting Influence on Long-Term Executive Functioning After Moderate to Severe Early Childhood Traumatic Brain Injury: An Exploratory Study — Kurowski et al., The Journal of head trauma rehabilitation (paywalled)
- saliva
- dna-genotek-oragene
- pediatric
- genotyping
2017
In an Irish cohort of 1516 participants, the rs2910164 variant in MIR146A was significantly associated with differentiated thyroid cancer (per allele OR = 1.59, P = 0.002) but not breast cancer. DNA extracted from saliva collected using Oragene 575 kits yielded successful genotyping, supporting decentralised screening for cancer predisposition.
Investigating the association of rs2910164 with cancer predisposition in an Irish cohort — McVeigh et al., Endocrine connections
- saliva
- dna-genotek-oragene
- genotyping
2017
Among 148 Marshallese adults recruited through community-based participatory research, 95.5% provided a 2 mL saliva specimen using an Oragene DNA self-collection kit and 96.6% agreed to future contact, demonstrating high acceptability of patient-centric, decentralised genetic sampling in an underserved population with disproportionate type 2 diabetes burden.
Leveraging community-based participatory research capacity to recruit Pacific Islanders into a genetics study — McElfish et al., Journal of community genetics (paywalled)
- saliva
- dna-genotek-oragene
- self-collection
- acceptability
- genotyping
2016
With Oragene DISCOVER self-collection, saliva SNP-array genotypes matched blood at 99.996% concordance; about 6% of samples failed on low yield, so an occasional repeat should be expected.
Saliva DNA quality and genotyping efficiency in a predominantly elderly population — Gudiseva et al., BMC Medical Genomics
- dna
- venous-agreement
- stabilised
- blood
- dna-genotek-oragene
- validation
- saliva
- genotyping
2016
An evaluation of five saliva collection kits found that only Norgen and Stratec achieved DNA yields comparable to Oragene of 30 µg or more, whilst Oasis was the easiest for sample collection. All commercial kits outperformed an in-house protocol, offering viable, cost-effective options for decentralised sampling.
Comparative Study of Five Commercially Available Saliva Collection Kits for DNA Extraction — Deeley et al., Clinical laboratory (paywalled)
- dna-genotek-oragene
- liquid
- validation
- saliva
- genotyping
2016
The study identified fungiform papillae biopsy as the optimal method for RNA integrity, though saliva techniques like the Oragene kit successfully amplified a subset of taste genes as a non-invasive alternative.
A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue — Archer et al., PloS one
- saliva
- dna-genotek-oragene
2016
Saliva collected with the Oragene DISCOVER kit from 30 patients with pheochromocytoma or paraganglioma produced DNA concentrations comparable to paired venous blood, though with slightly lower purity from protein contamination. Saliva is a viable alternative to blood for germline screening of SDHB and SDHD mutations in this population.
SDH Subunit Mutation Status in Saliva: Genetic Testing in Patients with Pheochromocytoma — Osinga et al., Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme (paywalled)
- saliva
- dna-genotek-oragene
- venous-agreement
- genotyping
2015
The DNA Genotek Oragene RNA assay yielded approximately four times more RNA from neonatal saliva than the Qiagen RNeasy kit and showed stronger correlation with RT-qPCR performance, though it required nearly twice the extraction time; RNA integrity was comparable between the two systems.
Comparative performance analyses of commercially available products for salivary collection and nucleic acid processing in the newborn — Maron & Johnson, Biotechnic & histochemistry : official publication of the Biological Stain Commission (paywalled)
- saliva
- stabilised
- dna-genotek-oragene
- pediatric
2014
A US survey of 3,400 adults aged 62 to 90 collected up to 15 biomeasures at home, including saliva, dried blood spots and urine, with Salivette and Oragene among the devices used, which shows that several patient-centric sampling methods can run together in home-based research.
Continuity and innovation in the data collection protocols of the second Wave of the National Social Life, Health, and Aging Project — Jaszczak et al., The journals of gerontology. Series B, Psychological sciences and social sciences (paywalled)
- actigraphy
- multimodal
- blood
- dbs
- cortisol
- dna-genotek-oragene
- self-collection
- dried
- bd-microtainer
- hormones
- liquid
- urine
- saliva
- biomarkers
- genotyping
2014
DNA concentration from whole saliva correlates positively with age, so children yield significantly less DNA than adults. This means saliva collection protocols for genetic studies must be adjusted for paediatric populations to ensure adequate DNA quantity for genotyping.
The effect of age on DNA concentration from whole saliva: implications for the standard isolation method — Gassó et al., American journal of human biology : the official journal of the Human Biology Council (paywalled)
- saliva
- dna-genotek-oragene
- validation
- pediatric
- genotyping
2014
This study genotyped two oestrogen receptor gene variants in DNA from saliva samples of Serbian women and found no association with premature ovarian failure. The use of Oragene kits for saliva collection shows a practical method for obtaining DNA in genetic research on reproductive conditions, though these specific markers appear uninformative in this population.
Lack of association between ESR1 gene polymorphisms and premature ovarian failure in Serbian women — Li et al., Climacteric : the journal of the International Menopause Society (paywalled)
- saliva
- dna-genotek-oragene
- fertility
- genotyping
2012
Oragene-stabilised saliva DNA agreed with blood at 98.7% of called markers on a pharmacogenetic array; saliva's lower overall call rate tracked its variable human-DNA content.
Genotyping Performance between Saliva and Blood-Derived Genomic DNAs on the DMET Array: A Comparison — Hu et al., PLoS ONE
- dna
- stabilised
- blood
- dna-genotek-oragene
- validation
- saliva
- genotyping
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