Integrating Microsampling in Human Biomonitoring: Methodologies, Regulatory Frameworks, and Case Study Insights

In 20 adults, CRP measured in urine and saliva strongly correlated with serum CRP (Spearman rho 0.886) and was significantly elevated in participants with systemic inflammation compared with controls, suggesting these noninvasive matrices could replace venipuncture for monitoring inflammatory status in remote or at-home settings.

Multimodal Noninvasive Assessment of C-Reactive Protein for Systemic Inflammation in Adults: Cross-Sectional Study — Shim et al., JMIR formative research · source ↗

• venous-agreement
• acceptability
• multimodal
• sweat
• stool
• digital-biomarker
• urine
• saliva
• biomarkers

The study validated a commercial ELISA for measuring measles-specific IgG in capillary dried blood spots from children, finding 100% sensitivity and 96.8% specificity against matched venous serum with 92% overall agreement, then applied the method to 1,588 field-collected samples in Mexico and Nicaragua. The procedure was acceptable to surveyors and participants, showing that dried blood spots are a feasible and accurate means of assessing population immunity to measles in low-resource settings.

Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings — Colson et al., Journal of medical virology (paywalled) · source ↗

• blood
• dried
• capillary
• dbs
• validation
• venous-agreement
• acceptability
• pediatric
• serology

A US survey of 3,400 adults aged 62 to 90 collected up to 15 biomeasures at home, including saliva, dried blood spots and urine, with Salivette and Oragene among the devices used, which shows that several patient-centric sampling methods can run together in home-based research.

Continuity and innovation in the data collection protocols of the second Wave of the National Social Life, Health, and Aging Project — Jaszczak et al., The journals of gerontology. Series B, Psychological sciences and social sciences (paywalled) · source ↗

• actigraphy
• multimodal
• blood
• dbs
• cortisol
• dna-genotek-oragene
• self-collection
• dried
• bd-microtainer
• hormones
• liquid
• urine
• saliva
• biomarkers
• genotyping

Frequent dried blood spot sampling detected early changes in beta-cell function more effectively than mixed-meal tolerance tests or urine ratios, supporting its potential as a decentralised monitoring tool for clinical trials.

Serial Dried Blood Spot C-Peptide Sampling, but Not Urine C-Peptide-to-Creatinine Ratio, Detects Early Preservation of β-Cell Function in New-Onset Type 1 Diabetes: Experience From the USTEKID Trial — Dunseath et al., Diabetes care (paywalled) · source ↗

• dct
• blood
• dbs
• dried
• pediatric
• urine
• biomarkers

In a 20-person proof-of-concept pilot among veterans with HIV, remote self-collection of dried blood spots for phosphatidylethanol monitoring was feasible and acceptable, with high return and analysis rates and positive feedback; a small first step toward decentralised alcohol biomarker monitoring rather than proof of it.

Remote alcohol biomarker monitoring to promote drinking reductions among people with HIV: A proof-of-concept pilot study — Sheinfil et al., Journal of substance use and addiction treatment (paywalled) · source ↗

• acceptability
• multimodal
• blood
• rpm
• dbs
• self-collection
• dried
• biomarkers