Evaluation of Patient-Centric Sample Collection Technologies for Pharmacokinetic Assessment of Large and Small Molecules

In 55 patients, four approaches to convert dried capillary blood concentrations to plasma equivalents were evaluated. All methods except haematocrit-based conversion yielded acceptable analytical and clinical agreement for paracetamol and metabolites, supporting the reliability of capillary microsampling for decentralised therapeutic drug monitoring.

Comparative Evaluation of Approaches to Convert Microsampled Capillary Blood Concentrations to Plasma Concentrations: Paracetamol and Metabolites as a Case Study — Boffel et al., The AAPS journal (paywalled) · source ↗

• vams
• dct
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• validation

This review found that volumetric microsampling devices, such as Mitra, HemaPEN, HemaXis DB10, and Tasso-M20, overcome haematocrit bias, whilst Telimmune plasma separation cards enable direct plasma collection without centrifugation. These technologies support patient-centric, decentralised sampling, though the authors note that clinical validation remains limited across different drugs and patient populations.

A Review of Blood-Based Microsampling Devices for Patient-Centered Application in Therapeutic Drug Monitoring — Huhn & Scherf-Clavel, Therapeutic drug monitoring (paywalled) · source ↗

• vams
• volumetric
• acceptability
• neoteryx-mitra
• blood
• dbs
• tdm
• self-collection
• haematocrit
• dried
• capillary
• validation

Volumetric microsampling can enable patient-centred therapeutic drug monitoring with high patient preference and acceptable method validation for many drugs, but conversion from capillary to plasma and agreement with venous sampling varies by analyte and requires careful validation.

Volumetric Microsampling for Patient-Centric Therapeutic Drug Monitoring in Clinical Pharmacology: A Scoping Review — Tummala et al., Journal of clinical pharmacology (paywalled) · source ↗

• volumetric
• venous-agreement
• acceptability
• blood
• tdm
• self-collection
• haematocrit
• dried
• capillary
• validation

A validated LC-MS/MS method for everolimus using Mitra and Capitainer devices found excellent agreement between capillary microsamples and venous whole blood in 33 adult transplant recipients. The results showed high accuracy and negligible haematocrit effects, supporting the use of microsampling for decentralised therapeutic drug monitoring.

Therapeutic Drug Monitoring of Everolimus Using Volumetric Absorptive Microsampling and Quantitative Dried Blood Spot Methods with LC-MS/MS in Adult Solid Organ Transplant Recipients: An Analytical and Clinical Comparative Study — Kocur et al., Molecules · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• qdbs
• tdm
• haematocrit
• dried
• capillary
• immunosuppressants
• validation

The study validated a VAMS-based LC-MS/MS method for measuring sirolimus levels, showing it was clinically equivalent to the standard whole blood method and suitable for therapeutic drug monitoring in paediatric transplant patients, supporting the use of decentralised microsampling for this purpose.

Personalization of pharmacotherapy with sirolimus based on volumetric absorptive microsampling (VAMS) in pediatric renal transplant recipients-from LC-MS/MS method validation to clinical application — Kocur et al., Pharmacological reports : PR · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• pediatric
• immunosuppressants
• validation