Volumetric Absorptive Microsampling of Saliva for Pharmacokinetic Evaluation of Mycophenolic Acid and Its Glucuronide Metabolite in Pediatric Renal Transplant Recipients: Bioanalytical Method Validation and Clinical Feasibility Evaluation

The study validated a VAMS-based LC-MS/MS method for measuring sirolimus levels, showing it was clinically equivalent to the standard whole blood method and suitable for therapeutic drug monitoring in paediatric transplant patients, supporting the use of decentralised microsampling for this purpose.

Personalization of pharmacotherapy with sirolimus based on volumetric absorptive microsampling (VAMS) in pediatric renal transplant recipients-from LC-MS/MS method validation to clinical application — Kocur et al., Pharmacological reports : PR · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• pediatric
• immunosuppressants
• validation

A study of 50 paediatric renal transplant recipients found that a validated VAMS LC-MS/MS method for tacrolimus showed high correlation with the reference method over 0.5–60 ng/mL, with minimal haematocrit effect.

Therapeutic Drug Monitoring of Tacrolimus Based on VAMS in Renal Transplant Pediatric Recipients — LC-MS/MS Method, Hematocrit Effect, and Clinical Application — Kocur et al., Pharmaceutics · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• pediatric
• immunosuppressants
• validation

The study developed and validated a rapid LC-MS/MS method for quantifying cefazolin in capillary whole blood collected via VAMS, plasma, and plasma ultrafiltrate, showing robust performance across matrices and successful application in a pediatric pilot cohort for therapeutic drug monitoring.

Rapid, robust LC-MS/MS quantification of cefazolin in whole blood microsamples, plasma, and plasma ultrafiltrate: Analytical method validation and preliminary clinical application in pediatric population — Kocur et al., Clinica chimica acta; international journal of clinical chemistry (paywalled) · source ↗

• vams
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• antimicrobials
• capillary
• pediatric
• validation

In matched clinical samples from rheumatoid arthritis, VAMS and DBS showed strong agreement for methotrexate polyglutamates (slopes 0.95-1.07; bias within -4.21% to 0.36%; SRCC ≥ 0.969), with up to 100% of total MTXPG results within ±20% limits; capillary microsampling agreed closely with whole blood but differed from red blood cells, indicating matrix-specific differences that must be accounted for when interpreting against RBC-based reference values.

Multi-Matrix LC-MS/MS Validation of Methotrexate Polyglutamates: Comparison of VAMS, DBS, and Conventional Blood Sampling in Rheumatoid Arthritis — Kocur et al., International journal of molecular sciences · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• dbs
• tdm
• dried
• capillary
• immunosuppressants
• validation

A validated LC-MS/MS method for everolimus using Mitra and Capitainer devices found excellent agreement between capillary microsamples and venous whole blood in 33 adult transplant recipients. The results showed high accuracy and negligible haematocrit effects, supporting the use of microsampling for decentralised therapeutic drug monitoring.

Therapeutic Drug Monitoring of Everolimus Using Volumetric Absorptive Microsampling and Quantitative Dried Blood Spot Methods with LC-MS/MS in Adult Solid Organ Transplant Recipients: An Analytical and Clinical Comparative Study — Kocur et al., Molecules · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• qdbs
• tdm
• haematocrit
• dried
• capillary
• immunosuppressants
• validation