Rapid, robust LC-MS/MS quantification of cefazolin in whole blood microsamples, plasma, and plasma ultrafiltrate: Analytical method validation and preliminary clinical application in pediatric population

The study validated a VAMS-based LC-MS/MS method for measuring sirolimus levels, showing it was clinically equivalent to the standard whole blood method and suitable for therapeutic drug monitoring in paediatric transplant patients, supporting the use of decentralised microsampling for this purpose.

Personalization of pharmacotherapy with sirolimus based on volumetric absorptive microsampling (VAMS) in pediatric renal transplant recipients-from LC-MS/MS method validation to clinical application — Kocur et al., Pharmacological reports : PR · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• pediatric
• immunosuppressants
• validation

A validated LC-MS method quantified multiple antibiotics from a single volumetric absorptive microsample, with stability up to 90 days for most analytes and minimal haematocrit effect except vancomycin, enabling pharmacokinetic and pharmacodynamic studies in decentralised settings.

Bioanalysis of six antibiotics from volumetric microsamples: a new tool for precision dosing in critically ill children — Takyi-Williams et al., Bioanalysis (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials
• haematocrit

A study of 50 paediatric renal transplant recipients found that a validated VAMS LC-MS/MS method for tacrolimus showed high correlation with the reference method over 0.5–60 ng/mL, with minimal haematocrit effect.

Therapeutic Drug Monitoring of Tacrolimus Based on VAMS in Renal Transplant Pediatric Recipients — LC-MS/MS Method, Hematocrit Effect, and Clinical Application — Kocur et al., Pharmaceutics · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• pediatric
• immunosuppressants
• validation

The honest counterpoint in children: VAMS predicted plasma vancomycin only modestly, venous/arterial VAMS was more accurate than capillary, and 29% of capillary samples were unusable for collection issues: collection technique and training matter as much as the assay.

Comparison of antibiotic sampling techniques: predicting plasma vancomycin from VAMS capillary versus venous/arterial whole blood — Downes et al., Open Forum Infect. Dis. · source ↗

• vams
• blood
• tdm
• haematocrit
• dried
• antimicrobials
• capillary
• pediatric
• validation

This review summarises liquid chromatography with tandem mass spectrometry assays using dried blood spots and volumetric absorptive microsampling for quantifying antimicrobial drugs in children, highlighting that VAMS reduces haematocrit bias and offers a viable alternative to traditional plasma sampling for therapeutic drug monitoring.

Microsampling Assays for Pharmacokinetic Analysis and Therapeutic Drug Monitoring of Antimicrobial Drugs in Children: A Critical Review — Moorthy et al., Therapeutic drug monitoring (paywalled) · source ↗

• blood
• dried
• vams
• dbs
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials
• haematocrit