Comparison of antibiotic sampling techniques: predicting plasma vancomycin from VAMS capillary versus venous/arterial whole blood

The study developed and validated a rapid LC-MS/MS method for quantifying cefazolin in capillary whole blood collected via VAMS, plasma, and plasma ultrafiltrate, showing robust performance across matrices and successful application in a pediatric pilot cohort for therapeutic drug monitoring.

Rapid, robust LC-MS/MS quantification of cefazolin in whole blood microsamples, plasma, and plasma ultrafiltrate: Analytical method validation and preliminary clinical application in pediatric population — Kocur et al., Clinica chimica acta; international journal of clinical chemistry (paywalled) · source ↗

• vams
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• antimicrobials
• capillary
• pediatric
• validation

A UPLC-MS/MS method for linezolid and metabolites in oral fluid and VAMS capillary blood satisfied ICH M10 validation requirements. Linezolid concentrations correlated strongly with venous plasma in both matrices, offering a non‑invasive option for therapeutic drug monitoring, whereas metabolites were systematically underestimated in VAMS and seldom detectable in oral fluid, restricting metabolite measurement.

Simultaneous quantification of linezolid and its metabolites (PNU-142300 and PNU-142586) in oral fluid and capillary blood by UPLC-MS/MS: method validation and clinical application using non-invasive sampling techniques — González-Berdullas et al., Journal of translational medicine (paywalled) · source ↗

• blood
• saliva
• dried
• capillary
• vams
• validation
• venous-agreement
• tdm
• antimicrobials
• haematocrit

The study compared quantitative dried blood spot (qDBS) and volumetric absorptive microsampling (VAMS) devices for measuring ganciclovir levels in capillary blood from pediatric renal transplant recipients. This comparison provides evidence for choosing between microsampling devices in therapeutic drug monitoring, supporting decentralised sampling approaches that reduce patient burden while maintaining analytical accuracy.

The comparison of two volumetric microsampling devices (qDBS and VAMS) for determining ganciclovir levels in capillary blood using the LC-MS/MS technique in pediatric renal transplant recipients — Kocur et al., European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (paywalled) · source ↗

• vams
• volumetric
• blood
• qdbs
• tdm
• dried
• antimicrobials
• capillary
• pediatric
• validation

In children with SLE, VAMS finger-prick capillary blood gave haematocrit-adjusted MPA and MPAG concentrations indistinguishable from plasma, and the AUC from VAMS derived plasma-equivalent concentrations matched plasma AUC with R2 0.97. This supports accurate pharmacokinetically guided dosing of MMF using only three timed capillary microsamples.

Volumetric Absorptive Microsampling Combined with Mass Spectrometry to Support Pharmacokinetically-Guided Precision Dosing of Mycophenolate Mofetil in Pediatric Lupus Nephritis Patients — Zhao et al., The journal of applied laboratory medicine (paywalled) · source ↗

• vams
• venous-agreement
• blood
• tdm
• haematocrit
• dried
• capillary
• pediatric
• immunosuppressants
• validation

The study validated a VAMS-based LC-MS/MS method for measuring sirolimus levels, showing it was clinically equivalent to the standard whole blood method and suitable for therapeutic drug monitoring in paediatric transplant patients, supporting the use of decentralised microsampling for this purpose.

Personalization of pharmacotherapy with sirolimus based on volumetric absorptive microsampling (VAMS) in pediatric renal transplant recipients-from LC-MS/MS method validation to clinical application — Kocur et al., Pharmacological reports : PR · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• pediatric
• immunosuppressants
• validation