Non-invasive Assessment of Vaccine-Induced HPV Antibodies via First-Void Urine
Pattyn et al.
The finding, in our words
Vaccine-induced HPV antibodies from cervicovaginal secretions were detectable in first-void urine and correlated with serum levels, so one self-collected urine sample can monitor both HPV infection and immunogenicity without a blood draw; the authors see promise for decentralised follow-up after vaccination.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
Adding polyethylene glycol to first-void urine before centrifugation markedly improved cell-free DNA recovery in the pellet, whereas low-speed centrifugation alone removed cellular DNA while keeping cell-free DNA in the supernatant. Pseudovirions pelleted reliably under all conditions, but the study revealed substantial variation between self-collected samples, highlighting the need for standardised protocols in decentralised HPV screening programmes.
In first-void urine collected with Colli-Pee, the extraction method influenced HPV and human DNA yield more than the collected volume did: standardising processing matters at least as much as standardising volume.
First-void urine samples showed significantly higher detection rates of HPV-specific IgG antibodies compared to midstream urine, confirming the necessity of capturing the initial stream. The study found no significant difference between 10 mL and 20 mL collection volumes, indicating flexibility in device specifications for self-collection.
The study found that testing for ASCL1/LHX8 DNA methylation in at-home collected first-void urine can detect most cervical cancers and high-grade precancers, supporting a fully molecular screening pathway without a clinical visit.
In a Belgian colposcopy referral cohort, a study found that 66.6% of women preferred home-based first-void urine self-sampling over physician-taken samples, and 96.6% found the Colli-Pee UCM FV-5010 device easy to use. This supports patient-centric, decentralised screening, though results are limited to this specific referral population.