Clinical performance of ASCL1/LHX8 DNA methylation on first-void urine to screen for cervical cancer

In first-void urine collected with Colli-Pee, the extraction method influenced HPV and human DNA yield more than the collected volume did: standardising processing matters at least as much as standardising volume.

Impact of Collection Volume and DNA Extraction Method on the Detection of Biomarkers and HPV DNA in First-Void Urine — Téblick et al., Molecules · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• liquid-biopsy
• liquid
• urine
• biomarkers

DNA methylation markers in first-void urine effectively detect high-grade cervical lesions in HPV-positive women, with results similar to clinician-collected samples in those over 30. This enables reliable cervical cancer screening using self-collected urine, reducing the need for invasive follow-up procedures in decentralised settings.

Evaluating DNA methylation markers and extended HPV genotyping in first-void urine for detecting high-grade cervical lesions in HPV-positive women: a cross-sectional study — Tranberg et al., BMC medicine · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• urine
• biomarkers
• genotyping

In a Belgian colposcopy referral cohort, a study found that 66.6% of women preferred home-based first-void urine self-sampling over physician-taken samples, and 96.6% found the Colli-Pee UCM FV-5010 device easy to use. This supports patient-centric, decentralised screening, though results are limited to this specific referral population.

Home-based urinary HPV self-sampling for the detection of cervical cancer precursor lesions: attitudes and preferences from Belgian females participating in the CASUS study — Hendrickx et al., Archives of public health = Archives belges de sante publique · source ↗

• colli-pee
• acceptability
• first-void
• self-collection
• hpv
• liquid-biopsy
• liquid
• urine

The review concludes that first-void urine is a feasible source for molecular biomarkers, such as host and viral gene methylation, to triage high-risk HPV infections, offering a non-invasive and preferred method for women that may improve screening adherence.

First-void urine: A potential biomarker source for triage of high-risk human papillomavirus infected women — Van Keer et al., European journal of obstetrics, gynecology, and reproductive biology (paywalled) · source ↗

• urine
• first-void
• self-collection
• validation
• hpv
• biomarkers
• liquid-biopsy

This meta-analysis found that self-collected urine samples for HPV testing had lower sensitivity for detecting high-grade lesions compared to physician-collected cervical specimens. The use of a first-void collection device such as Colli-Pee was associated with marginally higher sensitivity than general urine sampling.

Accuracy of HPV Testing Using Self-collected Urine Compared With Physician-collected Cervical Specimen for HSIL or Worse — Chen et al., Journal of lower genital tract disease (paywalled) · source ↗

• urine
• first-void
• colli-pee
• self-collection
• validation
• hpv
• sti