Concentration strategies for spiked and naturally present biomarkers in non-invasively collected first-void urine

In first-void urine collected with Colli-Pee, the extraction method influenced HPV and human DNA yield more than the collected volume did: standardising processing matters at least as much as standardising volume.

Impact of Collection Volume and DNA Extraction Method on the Detection of Biomarkers and HPV DNA in First-Void Urine — Téblick et al., Molecules · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• liquid-biopsy
• liquid
• urine
• biomarkers

Vaccine-induced HPV antibodies from cervicovaginal secretions were detectable in first-void urine and correlated with serum levels, so one self-collected urine sample can monitor both HPV infection and immunogenicity without a blood draw; the authors see promise for decentralised follow-up after vaccination.

Non-invasive Assessment of Vaccine-Induced HPV Antibodies via First-Void Urine — Pattyn et al., Frontiers in Immunology · source ↗

• first-void
• sti
• serology
• hpv
• liquid-biopsy
• liquid
• urine
• biomarkers

The review concludes that first-void urine is a feasible source for molecular biomarkers, such as host and viral gene methylation, to triage high-risk HPV infections, offering a non-invasive and preferred method for women that may improve screening adherence.

First-void urine: A potential biomarker source for triage of high-risk human papillomavirus infected women — Van Keer et al., European journal of obstetrics, gynecology, and reproductive biology (paywalled) · source ↗

• urine
• first-void
• self-collection
• validation
• hpv
• biomarkers
• liquid-biopsy

The study found that testing for ASCL1/LHX8 DNA methylation in at-home collected first-void urine can detect most cervical cancers and high-grade precancers, supporting a fully molecular screening pathway without a clinical visit.

Clinical performance of ASCL1/LHX8 DNA methylation on first-void urine to screen for cervical cancer — Van Keer et al., Communications medicine · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• liquid-biopsy
• urine
• biomarkers

In a Belgian colposcopy referral cohort, a study found that 66.6% of women preferred home-based first-void urine self-sampling over physician-taken samples, and 96.6% found the Colli-Pee UCM FV-5010 device easy to use. This supports patient-centric, decentralised screening, though results are limited to this specific referral population.

Home-based urinary HPV self-sampling for the detection of cervical cancer precursor lesions: attitudes and preferences from Belgian females participating in the CASUS study — Hendrickx et al., Archives of public health = Archives belges de sante publique · source ↗

• colli-pee
• acceptability
• first-void
• self-collection
• hpv
• liquid-biopsy
• liquid
• urine