Volumetric Absorptive Microsampling (VAMS) technology for IGF-1 quantification by automated chemiluminescent immunoassay in dried blood

The study developed and validated a LC-HRMS method for intact IGF-I from VAMS dried blood according to World Anti-Doping Agency requirements and showed agreement with serum measurements, supporting decentralised sampling for antidoping testing.

Use of capillary dried blood for quantification of intact IGF-I by LC-HRMS for antidoping analysis — Mongongu et al., Bioanalysis (paywalled) · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• doping
• blood
• dried
• capillary
• hormones
• validation
• biomarkers

A liquid chromatography-tandem mass spectrometry method was successfully validated for measuring androstenedione, 17α-hydroxyprogesterone, and 11-ketotestosterone in dried blood samples collected via volumetric absorptive microsampling. The dried samples remained stable at room temperature for up to a week and after postal transit, and plasma concentrations could be reliably estimated from the microsamples when adjusting for haematocrit.

Analytical validation of a dried blood microsampling method to measure androstenedione, 17α‑hydroxyprogesterone, and 11‑ketotestosterone for congenital adrenal hyperplasia monitoring — De Baets et al., Talanta (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• venous-agreement
• pediatric
• hormones
• haematocrit

Capillary samples collected using dried blood spots and Mitra microsamplers showed strong agreement with venous plasma for quantifying IgG antibodies against most vaccine-preventable diseases. Sensitivity was high for the majority of pathogens, though the study noted that antibody stability declined at room temperature over time, favouring cold storage for longer durations.

Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance — Ombati et al., Scientific reports (paywalled) · source ↗

• blood
• dried
• capillary
• vams
• dbs
• neoteryx-mitra
• validation
• venous-agreement
• serology

In matched clinical samples from rheumatoid arthritis, VAMS and DBS showed strong agreement for methotrexate polyglutamates (slopes 0.95-1.07; bias within -4.21% to 0.36%; SRCC ≥ 0.969), with up to 100% of total MTXPG results within ±20% limits; capillary microsampling agreed closely with whole blood but differed from red blood cells, indicating matrix-specific differences that must be accounted for when interpreting against RBC-based reference values.

Multi-Matrix LC-MS/MS Validation of Methotrexate Polyglutamates: Comparison of VAMS, DBS, and Conventional Blood Sampling in Rheumatoid Arthritis — Kocur et al., International journal of molecular sciences · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• dbs
• tdm
• dried
• capillary
• immunosuppressants
• validation

This study validated a method for measuring the alcohol biomarker phosphatidylethanol using two volumetric absorptive microsampling devices, finding that the Mitra device met all validation criteria and agreed with venous sampling. The Capitainer device was deemed suitable but showed reduced accuracy at higher concentrations, with a small proportional negative bias compared to venous results.

A validated method for capillary phosphatidylethanol 16:0/18:1 quantification with two different 10-µl volumetric absorptive microsample devices in the same setup — Andreassen et al., Journal of analytical toxicology (paywalled) · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• dbs
• dried
• toxicology
• capillary
• validation