Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance

In matched clinical samples from rheumatoid arthritis, VAMS and DBS showed strong agreement for methotrexate polyglutamates (slopes 0.95-1.07; bias within -4.21% to 0.36%; SRCC ≥ 0.969), with up to 100% of total MTXPG results within ±20% limits; capillary microsampling agreed closely with whole blood but differed from red blood cells, indicating matrix-specific differences that must be accounted for when interpreting against RBC-based reference values.

Multi-Matrix LC-MS/MS Validation of Methotrexate Polyglutamates: Comparison of VAMS, DBS, and Conventional Blood Sampling in Rheumatoid Arthritis — Kocur et al., International journal of molecular sciences · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• dbs
• tdm
• dried
• capillary
• immunosuppressants
• validation

This study validated a method for measuring the alcohol biomarker phosphatidylethanol using two volumetric absorptive microsampling devices, finding that the Mitra device met all validation criteria and agreed with venous sampling. The Capitainer device was deemed suitable but showed reduced accuracy at higher concentrations, with a small proportional negative bias compared to venous results.

A validated method for capillary phosphatidylethanol 16:0/18:1 quantification with two different 10-µl volumetric absorptive microsample devices in the same setup — Andreassen et al., Journal of analytical toxicology (paywalled) · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• dbs
• dried
• toxicology
• capillary
• validation

The study found very strong correlation between venous blood collection and capillary VAMS using Mitra devices for detecting SARS-CoV-2 spike antibodies, with samples remaining stable at room temperature for several months. This enables reliable at-home, patient-centric serological testing for large-scale community surveillance and vaccine studies.

Validation of at-home blood sampling for large scale, cost effective serological analysis for anti-viral antibody responses — Mayer et al., Journal of immunological methods (paywalled) · source ↗

• blood
• dried
• capillary
• vams
• neoteryx-mitra
• self-collection
• validation
• venous-agreement
• serology

This study found that dried blood microsamples, particularly those collected with the Mitra device, provided metabolic profiles comparable or superior to conventional plasma, supporting their use as a viable alternative for untargeted metabolomics.

Toward minimally invasive metabolomics: GC-MS metabolic fingerprints of dried blood microsamples in comparison to plasma — Marques de Sá E Silva et al., The Analyst (paywalled) · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• dbs
• metabolome
• dried
• capillary
• validation

In participants 11 months after SARS-CoV-2 infection, the nano-immunoassay achieved 98.33% sensitivity and 97.62% specificity on venous serum. When combined with dried capillary microsampling, sensitivity was 95.05% with Mitra, 61.11% with repurposed glucose strips and 83.16% with HemaXis (91.49% after automated extraction), with no loss of specificity, supporting home-based sampling for serology.

Clinical sensitivity and specificity of a high-throughput microfluidic nano-immunoassay combined with capillary blood microsampling for the identification of anti-SARS-CoV-2 Spike IgG serostatus — Michielin et al., PloS one · source ↗

• blood
• dried
• capillary
• vams
• neoteryx-mitra
• validation
• venous-agreement
• serology