Targeted DNA Methylation Analysis Facilitates Leukocyte Counts in Dried Blood Samples
Hubens et al.
The finding, in our words
Targeted DNA methylation analysis accurately estimated relative and absolute leukocyte subset counts from dried capillary blood collected with Mitra devices, showing strong correlation with conventional venous blood methods (r=0.72–0.97). This enables self-sampling by finger prick, facilitating easier access to leukocyte testing for patients with haematological disorders.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
A validated offline SPE-LC-MS/MS method using the Mitra microsampling device successfully quantified tacrolimus, cyclosporine A, tryptophan, kynurenine, and creatinine simultaneously. The method met EMA and FDA criteria, showing agreement between capillary and venous sampling, which could facilitate decentralised therapeutic drug monitoring and transplant follow-up through patient self-collection.
Nierychlewski et al., Therapeutic drug monitoring · source ↗
Capillary blood collection using volumetric absorptive microsampling demonstrated equivalent quantification of principal colonic polyphenol metabolites compared with matched venous plasma across a six-hour period following barley biscuit consumption. The technique successfully captured the pharmacokinetic profiles over 48 hours, confirming VAMS as a reliable alternative to venepuncture for dietary biomarker monitoring.
Cortijo-Alfonso et al., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (paywalled) · source ↗
Neoteryx Mitra sticks and Noviplex cards agreed more closely with venous plasma and gave more repeatable N-glycoprofiling results than dried blood spots, with relative deviance from plasma of 0.092 and 0.069 respectively compared with 0.674 for dried blood spots, and coefficient of variation values of 7.098 per cent and 4.831 per cent against 14.305 per cent for dried blood spots. The findings support these self-sampling devices for decentralised glycoanalysis, though the authors call for larger cohort validation.
The study developed and validated a LC-HRMS method for intact IGF-I from VAMS dried blood according to World Anti-Doping Agency requirements and showed agreement with serum measurements, supporting decentralised sampling for antidoping testing.
Mongongu et al., Bioanalysis (paywalled) · source ↗
Capillary C-peptide concentrations measured from dried blood spot and volumetric absorptive microsampling showed strong agreement with reference plasma samples. Both sampling methods maintained stability over 48 hours at room temperature, supporting their utility for remote monitoring of endogenous insulin secretion.
Jones et al., Journal of diabetes science and technology (paywalled) · source ↗