Quantitation of BCAA and BCKA in plasma and patient-centric dried blood microsamples in a clinical setting
Tierney et al.
The finding, in our words
A comparison of three patient-centric dried blood microsampling devices, paper DBS, Mitra and Tasso-M20, found strong to excellent correlation with traditional venous plasma for measuring branched-chain amino acids and ketoacids. Participants reported high acceptability and expressed a strong willingness to use these devices for decentralised self-collection.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
Untargeted metabolomic profiles from three blood microsampling devices aligned more closely with whole blood than with plasma, and all devices distinguished sex based on amino acids, lipids, and acylcarnitines. This validates that device choice can be tailored to the metabolites of interest for decentralised human biomonitoring.
In an untargeted metabolomics study, microsampling devices, particularly the Mitra and Capitainer, yielded metabolic profiles comparable or superior to plasma in feature number and intensity, and in the precision and stability of some metabolites. This supports their potential for large-scale, decentralised metabolic profiling, though the captured metabolite profile was application-dependent.
This study found that dried blood microsamples, particularly those collected with the Mitra device, provided metabolic profiles comparable or superior to conventional plasma, supporting their use as a viable alternative for untargeted metabolomics.
Capillary blood collection using volumetric absorptive microsampling demonstrated equivalent quantification of principal colonic polyphenol metabolites compared with matched venous plasma across a six-hour period following barley biscuit consumption. The technique successfully captured the pharmacokinetic profiles over 48 hours, confirming VAMS as a reliable alternative to venepuncture for dietary biomarker monitoring.
Neoteryx Mitra sticks and Noviplex cards agreed more closely with venous plasma and gave more repeatable N-glycoprofiling results than dried blood spots, with relative deviance from plasma of 0.092 and 0.069 respectively compared with 0.674 for dried blood spots, and coefficient of variation values of 7.098 per cent and 4.831 per cent against 14.305 per cent for dried blood spots. The findings support these self-sampling devices for decentralised glycoanalysis, though the authors call for larger cohort validation.