Dried blood spot validation of five immunosuppressants, without hematocrit correction, on two LC-MS/MS systems

Clinical validation for tacrolimus and mycophenolic acid monitoring showed that while volumetric absorptive microsampling met analytical criteria after concentration correction, conventional dried blood spots achieved superior adherence to strict clinical criteria, sample quality, and cost efficiency without significant haematocrit bias.

Comparison of conventional dried blood spots and volumetric absorptive microsampling for tacrolimus and mycophenolic acid determination — Paniagua-González et al., Journal of pharmaceutical and biomedical analysis (paywalled) · source ↗

• blood
• dried
• capillary
• vams
• dbs
• neoteryx-mitra
• self-collection
• validation
• venous-agreement
• tdm
• immunosuppressants
• economics
• haematocrit

In AML and CLL, 91% of VAMS venetoclax results fell within 20% of plasma after individualised haematocrit correction; in home sampling, 18 of 21 patients self-sampled independently and 76% of returned samples were analysable, which shows home microsampling is workable, though the authors ask for multicentre validation.

Clinical Validation of Venetoclax Volumetric Microsampling in Leukemia, with Whole-Blood-to-Plasma Conversion and Self-Microsampling Feasibility — Levens et al., Clinical Pharmacokinetics (paywalled) · source ↗

• vams
• venous-agreement
• blood
• dbs
• tdm
• self-collection
• haematocrit
• dried
• oncology
• capillary
• validation

VAMS microsampling found no significant difference from venous blood for tacrolimus in paediatric patients; samples were stable for 14 days, with average difference between paired at-home samples of 0.12 ± 0.94 ng/mL.

VAMS and parallel-reaction-monitoring mass spectrometry for tacrolimus trough measurements at home in paediatric heart-transplant patients — Zhao et al., J. Mass Spectrometry and Advances in the Clinical Lab · source ↗

• vams
• venous-agreement
• acceptability
• blood
• dbs
• tdm
• self-collection
• dried
• capillary
• pediatric
• immunosuppressants
• validation

Automated dried blood spot analysis achieved clinical and analytical acceptance criteria for tacrolimus, sirolimus, everolimus and cyclosporin A after haematocrit correction. This validates a method that could enable decentralised, patient-centric therapeutic drug monitoring for transplant recipients.

Application of a Fully Automated Dried Blood Spot Method for Therapeutic Drug Monitoring of Immunosuppressants: Another Step Toward Implementation of Dried Blood Spot Analysis — Deprez & Stove, Archives of pathology & laboratory medicine (paywalled) · source ↗

• blood
• dried
• dbs
• validation
• venous-agreement
• tdm
• immunosuppressants
• haematocrit

A new LC-MS/MS method quantifies four immunosuppressants from one 3.2 mm dried blood spot using cold-induced phase separation, achieving accurate results and favourable interchangeability with a certified whole blood method in 120 paired clinical samples, supporting decentralised therapeutic drug monitoring.

Quantification of immunosuppressants from one 3.2 mm dried blood spot by a novel cold-induced phase separation based LC-MS/MS method — Le et al., Analytica chimica acta (paywalled) · source ↗

• venous-agreement
• blood
• dbs
• tdm
• haematocrit
• dried
• immunosuppressants
• validation