2021 · Journal of pharmaceutical and biomedical analysis · paywalled
Determination of paracetamol and its metabolites via LC-MS/MS in dried blood volumetric absorptive microsamples: A tool for pharmacokinetic studies
Delahaye et al.
The finding, in our words
The authors developed and fully validated LC-MS/MS methods for paracetamol and four metabolites in plasma, whole blood and 10 microlitre VAMS dried blood microsamples, including assessment of haematocrit effects on VAMS recovery. Successful analysis of patient samples collected from both venous and capillary blood confirmed the methods are fit for purpose and can support future pharmacokinetic studies using decentralised microsampling.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
This review found that volumetric microsampling devices, such as Mitra, HemaPEN, HemaXis DB10, and Tasso-M20, overcome haematocrit bias, whilst Telimmune plasma separation cards enable direct plasma collection without centrifugation. These technologies support patient-centric, decentralised sampling, though the authors note that clinical validation remains limited across different drugs and patient populations.
Huhn & Scherf-Clavel, Therapeutic drug monitoring (paywalled) · source ↗
The study developed and validated a rapid LC-MS/MS method for quantifying cefazolin in capillary whole blood collected via VAMS, plasma, and plasma ultrafiltrate, showing robust performance across matrices and successful application in a pediatric pilot cohort for therapeutic drug monitoring.
Kocur et al., Clinica chimica acta; international journal of clinical chemistry (paywalled) · source ↗
In matched clinical samples from rheumatoid arthritis, VAMS and DBS showed strong agreement for methotrexate polyglutamates (slopes 0.95-1.07; bias within -4.21% to 0.36%; SRCC ≥ 0.969), with up to 100% of total MTXPG results within ±20% limits; capillary microsampling agreed closely with whole blood but differed from red blood cells, indicating matrix-specific differences that must be accounted for when interpreting against RBC-based reference values.
Kocur et al., International journal of molecular sciences · source ↗
A validated LC-MS/MS method for everolimus using Mitra and Capitainer devices found excellent agreement between capillary microsamples and venous whole blood in 33 adult transplant recipients. The results showed high accuracy and negligible haematocrit effects, supporting the use of microsampling for decentralised therapeutic drug monitoring.
A fully validated LC-MS method quantifies four direct oral anticoagulants and phenprocoumon from 10 µL capillary blood collected with Neoteryx Mitra VAMS devices. Samples remained stable for seven days at room temperature, showed no significant haematocrit effect, and offers a reliable approach for ambulatory therapeutic drug monitoring.
Opitz et al., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (paywalled) · source ↗