Determination of paracetamol and its metabolites via LC-MS/MS in dried blood volumetric absorptive microsamples: A tool for pharmacokinetic studies

This review found that volumetric microsampling devices, such as Mitra, HemaPEN, HemaXis DB10, and Tasso-M20, overcome haematocrit bias, whilst Telimmune plasma separation cards enable direct plasma collection without centrifugation. These technologies support patient-centric, decentralised sampling, though the authors note that clinical validation remains limited across different drugs and patient populations.

A Review of Blood-Based Microsampling Devices for Patient-Centered Application in Therapeutic Drug Monitoring — Huhn & Scherf-Clavel, Therapeutic drug monitoring (paywalled) · source ↗

• vams
• volumetric
• acceptability
• neoteryx-mitra
• blood
• dbs
• tdm
• self-collection
• haematocrit
• dried
• capillary
• validation

The study developed and validated a rapid LC-MS/MS method for quantifying cefazolin in capillary whole blood collected via VAMS, plasma, and plasma ultrafiltrate, showing robust performance across matrices and successful application in a pediatric pilot cohort for therapeutic drug monitoring.

Rapid, robust LC-MS/MS quantification of cefazolin in whole blood microsamples, plasma, and plasma ultrafiltrate: Analytical method validation and preliminary clinical application in pediatric population — Kocur et al., Clinica chimica acta; international journal of clinical chemistry (paywalled) · source ↗

• vams
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• antimicrobials
• capillary
• pediatric
• validation

A validated LC-MS/MS method for everolimus using Mitra and Capitainer devices found excellent agreement between capillary microsamples and venous whole blood in 33 adult transplant recipients. The results showed high accuracy and negligible haematocrit effects, supporting the use of microsampling for decentralised therapeutic drug monitoring.

Therapeutic Drug Monitoring of Everolimus Using Volumetric Absorptive Microsampling and Quantitative Dried Blood Spot Methods with LC-MS/MS in Adult Solid Organ Transplant Recipients: An Analytical and Clinical Comparative Study — Kocur et al., Molecules · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• qdbs
• tdm
• haematocrit
• dried
• capillary
• immunosuppressants
• validation

A fully validated LC-MS method quantifies four direct oral anticoagulants and phenprocoumon from 10 µL capillary blood collected with Neoteryx Mitra VAMS devices. Samples remained stable for seven days at room temperature, showed no significant haematocrit effect, and offers a reliable approach for ambulatory therapeutic drug monitoring.

Development and validation of a quantification method for direct oral anticoagulants from capillary blood using volumetric absorptive microsampling and online SPE-LC-MS — Opitz et al., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (paywalled) · source ↗

• blood
• dried
• capillary
• vams
• neoteryx-mitra
• validation
• tdm
• haematocrit

In 55 patients, four approaches to convert dried capillary blood concentrations to plasma equivalents were evaluated. All methods except haematocrit-based conversion yielded acceptable analytical and clinical agreement for paracetamol and metabolites, supporting the reliability of capillary microsampling for decentralised therapeutic drug monitoring.

Comparative Evaluation of Approaches to Convert Microsampled Capillary Blood Concentrations to Plasma Concentrations: Paracetamol and Metabolites as a Case Study — Boffel et al., The AAPS journal (paywalled) · source ↗

• vams
• dct
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• validation