2024 · European journal of medical research · open access
Concentration strategies for spiked and naturally present biomarkers in non-invasively collected first-void urine
Téblick et al.
The finding, in our words
Adding polyethylene glycol to first-void urine before centrifugation markedly improved cell-free DNA recovery in the pellet, whereas low-speed centrifugation alone removed cellular DNA while keeping cell-free DNA in the supernatant. Pseudovirions pelleted reliably under all conditions, but the study revealed substantial variation between self-collected samples, highlighting the need for standardised protocols in decentralised HPV screening programmes.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
In first-void urine collected with Colli-Pee, the extraction method influenced HPV and human DNA yield more than the collected volume did: standardising processing matters at least as much as standardising volume.
Vaccine-induced HPV antibodies from cervicovaginal secretions were detectable in first-void urine and correlated with serum levels, so one self-collected urine sample can monitor both HPV infection and immunogenicity without a blood draw; the authors see promise for decentralised follow-up after vaccination.
The review concludes that first-void urine is a feasible source for molecular biomarkers, such as host and viral gene methylation, to triage high-risk HPV infections, offering a non-invasive and preferred method for women that may improve screening adherence.
Van Keer et al., European journal of obstetrics, gynecology, and reproductive biology (paywalled) · source ↗
The study found that testing for ASCL1/LHX8 DNA methylation in at-home collected first-void urine can detect most cervical cancers and high-grade precancers, supporting a fully molecular screening pathway without a clinical visit.
Van Keer et al., Communications medicine · source ↗
In a Belgian colposcopy referral cohort, a study found that 66.6% of women preferred home-based first-void urine self-sampling over physician-taken samples, and 96.6% found the Colli-Pee UCM FV-5010 device easy to use. This supports patient-centric, decentralised screening, though results are limited to this specific referral population.
Hendrickx et al., Archives of public health = Archives belges de sante publique · source ↗