Assessment of Dried Serum Spots (DSS) and Volumetric-Absorptive Microsampling (VAMS) Techniques in Therapeutic Drug Monitoring of (Val)Ganciclovir-Comparative Study in Analytical and Clinical Practice

The study developed and validated a rapid LC-MS/MS method for quantifying cefazolin in capillary whole blood collected via VAMS, plasma, and plasma ultrafiltrate, showing robust performance across matrices and successful application in a pediatric pilot cohort for therapeutic drug monitoring.

Rapid, robust LC-MS/MS quantification of cefazolin in whole blood microsamples, plasma, and plasma ultrafiltrate: Analytical method validation and preliminary clinical application in pediatric population — Kocur et al., Clinica chimica acta; international journal of clinical chemistry (paywalled) · source ↗

• vams
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• antimicrobials
• capillary
• pediatric
• validation

This study validated a method for quantifying fluconazole using the Mitra device, demonstrating sufficient accuracy and precision for therapeutic drug monitoring in paediatric patients.

Determination of Fluconazole in Children in Small Blood Volumes Using Volumetric Absorptive Microsampling (VAMS) and Isocratic High-Performance Liquid Chromatography-Ultraviolet (HPLC-UV) Detection — Zimbelmann et al., Pharmaceutics · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials

A validated LC-MS method quantified multiple antibiotics from a single volumetric absorptive microsample, with stability up to 90 days for most analytes and minimal haematocrit effect except vancomycin, enabling pharmacokinetic and pharmacodynamic studies in decentralised settings.

Bioanalysis of six antibiotics from volumetric microsamples: a new tool for precision dosing in critically ill children — Takyi-Williams et al., Bioanalysis (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials
• haematocrit

Volumetric absorptive microsampling of whole blood did not provide a reliable basis for calculating meropenem plasma concentrations in critically ill children, so a validated low volume plasma method was required for paediatric therapeutic drug monitoring.

Developing a Method for Quantifying Meropenem in Children-Volumetric Adsorptive Microsampling Versus Plasma Sampling — Ramadan et al., Therapeutic drug monitoring (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• venous-agreement
• pediatric
• tdm
• antimicrobials

This review summarises liquid chromatography with tandem mass spectrometry assays using dried blood spots and volumetric absorptive microsampling for quantifying antimicrobial drugs in children, highlighting that VAMS reduces haematocrit bias and offers a viable alternative to traditional plasma sampling for therapeutic drug monitoring.

Microsampling Assays for Pharmacokinetic Analysis and Therapeutic Drug Monitoring of Antimicrobial Drugs in Children: A Critical Review — Moorthy et al., Therapeutic drug monitoring (paywalled) · source ↗

• blood
• dried
• vams
• dbs
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials
• haematocrit