2016 · Antimicrobial agents and chemotherapy · paywalled
Validation and Application of a Dried Blood Spot Ceftriaxone Assay
Page-Sharp et al.
The finding, in our words
The validation of a dried blood spot assay for ceftriaxone achieved a limit of quantification of 0.14 mg/L with strong correlation between DBS-predicted and measured plasma concentrations (r=0.95). The method showed acceptable thermal stability, retaining over 95% of initial concentrations for periods ranging from 14 hours to 11 months. This supports the use of self-collected DBS samples for therapeutic drug monitoring of antimicrobials in remote and resource-poor settings.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
The authors validated a dried blood spot assay for benznidazole that showed strong agreement with plasma concentrations (r²=0.8295) and proved stable at room temperature for more than one year. This microsampling approach enables therapeutic drug monitoring for Chagas disease in decentralised trials and remote settings, including paediatric populations.
Galindo Bedor et al., Antimicrobial agents and chemotherapy · source ↗
Miltefosine concentrations measured in dried blood spots showed excellent agreement with plasma (median ratio 0.99, Pearson's r=0.946) in patients with visceral leishmaniasis. The method demonstrated 97% extraction recovery, remained stable for 162 days at 37°C, and performed reliably across haematocrit levels of 20–35%, offering a valid and practical alternative to venous sampling for therapeutic drug monitoring in decentralised settings.
Kip et al., Antimicrobial agents and chemotherapy · source ↗
Parsons et al. validated a quantitative dried blood spot assay for rifapentine that remained stable for 11 weeks at ambient temperature and showed good agreement with plasma concentrations after haematocrit correction, offering a low-volume sampling approach suitable for therapeutic drug monitoring in international and paediatric tuberculosis trials.
Parsons et al., Antimicrobial agents and chemotherapy (paywalled) · source ↗
In AML and CLL, 91% of VAMS venetoclax results fell within 20% of plasma after individualised haematocrit correction; in home sampling, 18 of 21 patients self-sampled independently and 76% of returned samples were analysable, which shows home microsampling is workable, though the authors ask for multicentre validation.
Levens et al., Clinical Pharmacokinetics (paywalled) · source ↗
The study validated an LC-MS/MS method for eight antiepileptic drugs and two metabolites in dried blood spot and VAMS formats, with satisfactory analytical performance and stability, and was the first to include the oxcarbazepine metabolite DHCB. In 80 paired patient samples, microsampling concentrations showed promising correlation with plasma, supporting decentralised therapeutic drug monitoring of antiepileptic treatment.
Cobo-Golpe et al., Journal of analytical toxicology · source ↗