2018 · Antimicrobial agents and chemotherapy · open access
Dried Blood Spot Technique-Based Liquid Chromatography-Tandem Mass Spectrometry Method as a Simple Alternative for Benznidazole Pharmacokinetic Assessment
Galindo Bedor et al.
The finding, in our words
The authors validated a dried blood spot assay for benznidazole that showed strong agreement with plasma concentrations (r²=0.8295) and proved stable at room temperature for more than one year. This microsampling approach enables therapeutic drug monitoring for Chagas disease in decentralised trials and remote settings, including paediatric populations.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
A validated high-throughput LC-MS/MS assay for piperaquine from dried blood spots achieved 54-72% recovery with <9% relative standard deviation and a quantification limit of 3 ng/mL, enabling detection for 4-8 weeks post-dose. The method showed minimal haematocrit interference and is suitable for therapeutic drug monitoring of antimalarial treatment in resource-limited settings and pediatric populations.
Blessborn et al., Journal of mass spectrometry and advances in the clinical lab · source ↗
This review summarises liquid chromatography with tandem mass spectrometry assays using dried blood spots and volumetric absorptive microsampling for quantifying antimicrobial drugs in children, highlighting that VAMS reduces haematocrit bias and offers a viable alternative to traditional plasma sampling for therapeutic drug monitoring.
Moorthy et al., Therapeutic drug monitoring (paywalled) · source ↗
The validated LC-MS/MS method showed similar pharmacokinetic profiles for albendazole and its metabolites across plasma, whole blood, dried blood spots and Mitra microsamples from hookworm-infected adolescents, supporting microsampling for paediatric pharmacokinetic studies. Mitra extraction proved more robust than dried blood spots during validation and is recommended for future albendazole pharmacokinetic work, despite higher albendazole sulfone concentrations observed in both microsampling devices compared with wet matrices.
Schulz et al., Antimicrobial agents and chemotherapy (paywalled) · source ↗
A VAMS-LC-MS/MS method was developed and validated for measuring four antibiotics in 10 microlitres of human blood, showing that VAMS provided accurate quantification unaffected by haematocrit, unlike dried blood spots. The method was applied to paediatric patient samples, supporting its use for therapeutic drug monitoring in children.
Barco et al., Journal of pharmaceutical and biomedical analysis (paywalled) · source ↗
Miltefosine concentrations measured in dried blood spots showed excellent agreement with plasma (median ratio 0.99, Pearson's r=0.946) in patients with visceral leishmaniasis. The method demonstrated 97% extraction recovery, remained stable for 162 days at 37°C, and performed reliably across haematocrit levels of 20–35%, offering a valid and practical alternative to venous sampling for therapeutic drug monitoring in decentralised settings.
Kip et al., Antimicrobial agents and chemotherapy · source ↗