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2021 · Life · open access

Use of Quantitative Dried Blood Spots to Evaluate the Post-Vaccination Level of Neutralizing Antibodies against SARS-CoV-2

Marchand et al.

The finding, in our words

A quantitative dried blood spot device (Capitainer qDBS) combined with the Roche Elecsys anti-SARS-CoV-2 S immunoassay tracked anti-spike antibody development after two doses of the Pfizer vaccine, with the second dose producing a roughly hundredfold stimulation that peaked at two weeks and fell to 65 percent by three months. The bias between plasma and DBS was variable across both a 14-volunteer vaccination cohort and a 200-patient clinical cohort, yet DBS proved sufficiently sensitive for prolonged immune-status monitoring without venous draws.

A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.

Read the source ↗DOI 10.3390/life11111125PubMed 34833001

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Papers that share these labels

  1. 2026

    The study validated a semi-quantitative protocol for Epstein-Barr virus serology using dried blood spots, demonstrating 98.8% sensitivity and 96.5% specificity compared to paired venous serum samples. The successful validation in a self-sampling cohort confirms the suitability of this patient-centric microsampling approach for large-scale seroprevalence studies and decentralised trials.

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  2. 2024

    Self-collected dried blood spots analysed on the Roche Elecsys platform gave quantitative anti-spike antibody results that agreed closely with paired venous samples, with sensitivity 96.63% and specificity 97.81% and a conversion formula across 0.018 to 250 U/mL, enabling accurate tracking of titre changes in longitudinal cohorts after vaccination.

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  3. 2023

    Dried blood spot eluates showed high agreement with serum for SARS-CoV-2 IgG against Spike and Nucleocapsid (r≈0.96; qualitative φ≈0.98–1.0) and remained stable after 6–8 hours of storage, supporting use of capillary DBS for serosurveys and decentralised testing.

    Dried blood spot eluates are suitable for testing of SARS-CoV-2 IgG antibodies targeting Spike protein 1 and Nucleocapsid protein — Guttmann et al., Biochemistry and biophysics reports · source ↗

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  4. 2023

    In 1070 young adults, dried blood spot eluates correlated strongly with serum for anti-spike IgGAM and total IgG, IgA and IgM; labDBS achieved 82.0% sensitivity and 98.2% specificity and ssDBS 86.1% sensitivity and 96.7% specificity versus serum, with minimal difference between self and investigator collection.

    Validation of dried blood spot sampling for detecting SARS-CoV-2 antibodies and total immunoglobulins in a large cohort of asymptomatic young adults — Ferentinos et al., Journal of immunological methods · source ↗

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  5. 2022

    Dried blood spot sampling achieved high sensitivity and specificity for SARS-CoV-2 antibody detection with strong correlation to paired serum, enabling virtually painless, decentralised specimen collection in community and remote settings where venepuncture is difficult.

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