2017 · Annals of oncology : official journal of the European Society for Medical Oncology · paywalled
Tracking a CAD-ALK gene rearrangement in urine and blood of a colorectal cancer patient treated with an ALK inhibitor
Siravegna et al.
The finding, in our words
Urine trans-renal DNA and plasma circulating tumour DNA showed concordant dynamics that paralleled clinical course in a metastatic colorectal cancer patient treated with an ALK inhibitor. Urine-based detection of the CAD-ALK rearrangement anticipated radiological confirmation of disease progression, demonstrating that self-collected urine can non-invasively monitor tumour evolution and emerging resistance mutations during therapy.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
This review summarises how emerging HPV detection methods including CRISPR/Cas systems, droplet digital PCR and isothermal amplification, combined with self-collected vaginal swabs and liquid biopsy from urine or blood, could improve screening acceptability and enable point-of-care testing compared with conventional approaches that require invasive sampling and centralised laboratory infrastructure.
Shi et al., Frontiers in cellular and infection microbiology · source ↗
Urine-based liquid biopsy detecting trans-renal cell-free tumor DNA provides a non-invasive alternative to blood sampling, enabling frequent home self-collection and mail-in monitoring of cancer biomarker kinetics.
The authors developed an integrated sequencing workflow that simultaneously profiles copy number aberrations and a three-gene methylation panel from home-collected urine cell-free DNA, achieving high diagnostic accuracy for both primary and recurrent bladder cancer in a cohort of 89 participants, which supports the feasibility of decentralised cancer detection from self-collected urine samples.
Beijert et al., JCO precision oncology (paywalled) · source ↗
An eight-millilitre sample of first-morning urine self-collected without prostatic massage yields reproducible microbial profiles using PathoChip microarray. The workflow identified Streptococcaceae enrichment in higher-grade prostate cancer, supporting its potential for decentralised, non-invasive screening, though larger validation cohorts are needed.
Mello-Grand et al., The journal of liquid biopsy (paywalled) · source ↗
In a decentralised Eswatini clinic, molecular testing on the Xpert platform of self-collected urine and swabs found a 31.7% prevalence of Chlamydia, Gonorrhoea, or Trichomonas, with 20.8% of these asymptomatic. Although limited by a cross-sectional design, this shows the feasibility of patient-centric sampling for sexually transmitted infection screening in resource-limited settings.
Kerschberger et al., Tropical medicine & international health : TM & IH · source ↗