Application of PathoChip to urine-derived nucleic acids for broad microbial profiling in men with suspected prostate cancer: setup of a methodological workflow and pilot feasibility study
Mello-Grand et al.
The finding, in our words
An eight-millilitre sample of first-morning urine self-collected without prostatic massage yields reproducible microbial profiles using PathoChip microarray. The workflow identified Streptococcaceae enrichment in higher-grade prostate cancer, supporting its potential for decentralised, non-invasive screening, though larger validation cohorts are needed.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
The authors developed an integrated sequencing workflow that simultaneously profiles copy number aberrations and a three-gene methylation panel from home-collected urine cell-free DNA, achieving high diagnostic accuracy for both primary and recurrent bladder cancer in a cohort of 89 participants, which supports the feasibility of decentralised cancer detection from self-collected urine samples.
Beijert et al., JCO precision oncology (paywalled) · source ↗
In a Belgian colposcopy referral cohort, a study found that 66.6% of women preferred home-based first-void urine self-sampling over physician-taken samples, and 96.6% found the Colli-Pee UCM FV-5010 device easy to use. This supports patient-centric, decentralised screening, though results are limited to this specific referral population.
Hendrickx et al., Archives of public health = Archives belges de sante publique · source ↗
This review summarises how emerging HPV detection methods including CRISPR/Cas systems, droplet digital PCR and isothermal amplification, combined with self-collected vaginal swabs and liquid biopsy from urine or blood, could improve screening acceptability and enable point-of-care testing compared with conventional approaches that require invasive sampling and centralised laboratory infrastructure.
Shi et al., Frontiers in cellular and infection microbiology · source ↗
Urine-based liquid biopsy detecting trans-renal cell-free tumor DNA provides a non-invasive alternative to blood sampling, enabling frequent home self-collection and mail-in monitoring of cancer biomarker kinetics.
In first-void urine collected with Colli-Pee, the extraction method influenced HPV and human DNA yield more than the collected volume did: standardising processing matters at least as much as standardising volume.