[Salivary steroid profile investigation by high performance liquid chromatography-tandem mass spectrometry in children with congenital adrenal hyperplasia]

During ACTH stimulation, salivary cortisol correlated only weakly with serum and gave modest accuracy: saliva does not reliably replace serum cortisol for adrenal insufficiency. A concrete limit.

Reliability of Salivary versus Serum Cortisol for Diagnosing Adrenal Insufficiency in Children — Ciancia et al., Children · source ↗

• venous-agreement
• blood
• cortisol
• pediatric
• hormones
• liquid
• validation
• saliva

A liquid chromatography-tandem mass spectrometry method was successfully validated for measuring androstenedione, 17α-hydroxyprogesterone, and 11-ketotestosterone in dried blood samples collected via volumetric absorptive microsampling. The dried samples remained stable at room temperature for up to a week and after postal transit, and plasma concentrations could be reliably estimated from the microsamples when adjusting for haematocrit.

Analytical validation of a dried blood microsampling method to measure androstenedione, 17α‑hydroxyprogesterone, and 11‑ketotestosterone for congenital adrenal hyperplasia monitoring — De Baets et al., Talanta (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• venous-agreement
• pediatric
• hormones
• haematocrit

This study used home self-collected saliva samples to successfully monitor cytokines and cortisol in mothers and infants, finding that a community singing intervention significantly reduced salivary interleukin-6 levels in mothers with postnatal depression.

Community singing intervention reduces maternal salivary cytokines and enhances maternal-infant cortisol synchrony in postnatal depression: biological findings from the SHAPER-PND trial — Kirkpatrick et al., Brain, behavior, and immunity (paywalled) · source ↗

• dct
• cortisol
• self-collection
• pediatric
• hormones
• liquid
• saliva
• biomarkers

Self-collected salivary progesterone and the ratio of estradiol to progesterone showed strong agreement with serum levels, supporting the use of saliva as a noninvasive matrix for monitoring the menstrual cycle.

Salivary estradiol and progesterone across the menstrual cycle: Association and agreement with serum concentrations — Goldenstein et al., Physiological reports · source ↗

• venous-agreement
• blood
• self-collection
• fertility
• hormones
• saliva

This study found that salivary 11-dehydrodexamethasone correlates strongly with serum dexamethasone, unlike salivary dexamethasone which shows poor agreement. This validation suggests patients could self-collect saliva samples at home to verify dexamethasone absorption for the overnight suppression test.

The Utility of Salivary 11-Dehydrodexamethasone as a Marker of Dexamethasone Absorption — Marshall et al., The Journal of clinical endocrinology and metabolism (paywalled) · source ↗

• venous-agreement
• tdm
• cortisol
• self-collection
• hormones
• liquid
• validation
• saliva