Isolation of salivary cell-free DNA for cancer detection

A US survey of 3,400 adults aged 62 to 90 collected up to 15 biomeasures at home, including saliva, dried blood spots and urine, with Salivette and Oragene among the devices used, which shows that several patient-centric sampling methods can run together in home-based research.

Continuity and innovation in the data collection protocols of the second Wave of the National Social Life, Health, and Aging Project — Jaszczak et al., The journals of gerontology. Series B, Psychological sciences and social sciences (paywalled) · source ↗

• actigraphy
• multimodal
• blood
• dbs
• cortisol
• dna-genotek-oragene
• self-collection
• dried
• bd-microtainer
• hormones
• liquid
• urine
• saliva
• biomarkers
• genotyping

In people at high risk of nasopharyngeal carcinoma, saliva showed decreased EBV load and increased EBV DNA methylation compared with controls, and a stepwise strategy that adds saliva testing to serum EBV antibodies improved triage efficiency.

Evaluation of Epstein-Barr virus DNA methylation and load in saliva in the management of individuals at high risk of nasopharyngeal carcinoma — Zheng et al., Biomarker research · source ↗

• serology
• liquid-biopsy
• liquid
• saliva
• biomarkers

Salivary HPV DNA testing achieved 91.7% sensitivity and 100% specificity against oropharyngeal swab in patients with oral cavity cancer, demonstrating potential for non-invasive self-collection screening programmes and long-term monitoring of HPV-driven disease.

Application of a Saliva-Based Liquid Biopsy for the Detection of HPV in Patients With Oral Cavity Squamous Cell Carcinoma (OCSCC) — Bulfoni et al., Head & neck · source ↗

• self-collection
• hpv
• liquid-biopsy
• liquid
• validation
• saliva

A saliva-derived transcriptomic signature using support vector machine learning detected gastric cancer with an AUC of 0.87, sensitivity of 79% and specificity of 70%. Tissue-based gene expression models performed poorly when applied to saliva, indicating that saliva-specific biomarker panels are needed for effective non-invasive gastric cancer screening.

Saliva-derived transcriptomic signature for gastric cancer detection using machine learning and leveraging publicly available datasets — Lopes et al., Scientific reports · source ↗

• self-collection
• liquid-biopsy
• liquid
• saliva
• biomarkers

Self-collected oral sponge sampling found high accuracy for HPV DNA detection in oral cancer patients, with 100% specificity, 85.7% sensitivity and an AUC of 0.93, compared to oral rinse. The authors note larger cohorts are required to confirm performance.

Human Papilloma Virus (HPV) Detection in Oral Rinse vs. Oral Sponge: A Preliminary Accuracy Report in Oral Cancer Patients — Panzarella et al., Cancers · source ↗

• self-collection
• hpv
• liquid
• saliva
• genotyping