Development and validation of ivermectin quantification method in volumetric absorptive microsampling using liquid chromatography-tandem mass spectrometry

The study developed and validated a rapid LC-MS/MS method for quantifying cefazolin in capillary whole blood collected via VAMS, plasma, and plasma ultrafiltrate, showing robust performance across matrices and successful application in a pediatric pilot cohort for therapeutic drug monitoring.

Rapid, robust LC-MS/MS quantification of cefazolin in whole blood microsamples, plasma, and plasma ultrafiltrate: Analytical method validation and preliminary clinical application in pediatric population — Kocur et al., Clinica chimica acta; international journal of clinical chemistry (paywalled) · source ↗

• vams
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• antimicrobials
• capillary
• pediatric
• validation

A validated LC-MS method quantified multiple antibiotics from a single volumetric absorptive microsample, with stability up to 90 days for most analytes and minimal haematocrit effect except vancomycin, enabling pharmacokinetic and pharmacodynamic studies in decentralised settings.

Bioanalysis of six antibiotics from volumetric microsamples: a new tool for precision dosing in critically ill children — Takyi-Williams et al., Bioanalysis (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials
• haematocrit

This review summarises liquid chromatography with tandem mass spectrometry assays using dried blood spots and volumetric absorptive microsampling for quantifying antimicrobial drugs in children, highlighting that VAMS reduces haematocrit bias and offers a viable alternative to traditional plasma sampling for therapeutic drug monitoring.

Microsampling Assays for Pharmacokinetic Analysis and Therapeutic Drug Monitoring of Antimicrobial Drugs in Children: A Critical Review — Moorthy et al., Therapeutic drug monitoring (paywalled) · source ↗

• blood
• dried
• vams
• dbs
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials
• haematocrit

A VAMS-LC-MS/MS method was developed and validated for measuring four antibiotics in 10 microlitres of human blood, showing that VAMS provided accurate quantification unaffected by haematocrit, unlike dried blood spots. The method was applied to paediatric patient samples, supporting its use for therapeutic drug monitoring in children.

Volumetric adsorptive microsampling-liquid chromatography tandem mass spectrometry assay for the simultaneous quantification of four antibiotics in human blood: Method development, validation and comparison with dried blood spot — Barco et al., Journal of pharmaceutical and biomedical analysis (paywalled) · source ↗

• blood
• dried
• vams
• dbs
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials
• haematocrit

An LC-MS/MS method for miltefosine quantification using VAMS was successfully validated, showing good accuracy within ±10.8% and precision below 11.9%. Although VAMS reduced haematocrit-induced bias compared to traditional dried blood spots, recovery still declined at higher haematocrit levels.

Volumetric absorptive microsampling (VAMS) as an alternative to conventional dried blood spots in the quantification of miltefosine in dried blood samples — Kip et al., Journal of pharmaceutical and biomedical analysis (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• tdm
• antimicrobials
• haematocrit