2025 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · paywalled
Development and validation of an LC-MS/MS multiplex assay for the quantification of bedaquiline, n-desmethyl bedaquiline, linezolid, levofloxacin, and clofazimine in dried blood spots
Kenfack Teponnou et al.
The finding, in our words
An LC-MS/MS dried blood spot assay for five tuberculosis drugs achieved correlation coefficients of 0.87–0.99 against plasma methods, with more than 67% of samples within 20% of plasma values, meeting EMA reproducibility standards. This shows interchangeability with conventional sampling and supports decentralised therapeutic drug monitoring for rifampicin-resistant tuberculosis in low-resource settings.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
The study found that dried blood spot sampling showed good agreement with plasma concentrations for vancomycin and creatinine, with most differences within 20%, and patients preferred the finger prick method over venepuncture, supporting its use for decentralised therapeutic drug monitoring.
Hassanzai et al., The Journal of antimicrobial chemotherapy · source ↗
The validated LC-MS/MS method showed similar pharmacokinetic profiles for albendazole and its metabolites across plasma, whole blood, dried blood spots and Mitra microsamples from hookworm-infected adolescents, supporting microsampling for paediatric pharmacokinetic studies. Mitra extraction proved more robust than dried blood spots during validation and is recommended for future albendazole pharmacokinetic work, despite higher albendazole sulfone concentrations observed in both microsampling devices compared with wet matrices.
Schulz et al., Antimicrobial agents and chemotherapy (paywalled) · source ↗
The authors validated a dried blood spot assay for benznidazole that showed strong agreement with plasma concentrations (r²=0.8295) and proved stable at room temperature for more than one year. This microsampling approach enables therapeutic drug monitoring for Chagas disease in decentralised trials and remote settings, including paediatric populations.
Galindo Bedor et al., Antimicrobial agents and chemotherapy · source ↗
Miltefosine concentrations measured in dried blood spots showed excellent agreement with plasma (median ratio 0.99, Pearson's r=0.946) in patients with visceral leishmaniasis. The method demonstrated 97% extraction recovery, remained stable for 162 days at 37°C, and performed reliably across haematocrit levels of 20–35%, offering a valid and practical alternative to venous sampling for therapeutic drug monitoring in decentralised settings.
Kip et al., Antimicrobial agents and chemotherapy · source ↗
The validation of a dried blood spot assay for ceftriaxone achieved a limit of quantification of 0.14 mg/L with strong correlation between DBS-predicted and measured plasma concentrations (r=0.95). The method showed acceptable thermal stability, retaining over 95% of initial concentrations for periods ranging from 14 hours to 11 months. This supports the use of self-collected DBS samples for therapeutic drug monitoring of antimicrobials in remote and resource-poor settings.
Page-Sharp et al., Antimicrobial agents and chemotherapy (paywalled) · source ↗