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OpenSampling

2021 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · paywalled

Volumetric absorptive microsampling as alternative sampling technique for renal function assessment in the paediatric population using iohexol

Dhondt et al.

The finding, in our words

Volumetric absorptive microsampling (VAMS) achieved excellent correlation with conventional venous sampling for iohexol quantification and glomerular filtration rate (GFR) derivation in twenty children, showing minimal bias overall. Accuracy was lower for GFR values under 60 mL/min/1.73 m², but the method proved stable for 245 days and valid across a 20–60 % haematocrit range, offering a viable decentralised alternative for paediatric renal function assessment.

A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.

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  1. 2026

    A liquid chromatography-tandem mass spectrometry method was successfully validated for measuring androstenedione, 17α-hydroxyprogesterone, and 11-ketotestosterone in dried blood samples collected via volumetric absorptive microsampling. The dried samples remained stable at room temperature for up to a week and after postal transit, and plasma concentrations could be reliably estimated from the microsamples when adjusting for haematocrit.

    Analytical validation of a dried blood microsampling method to measure androstenedione, 17α‑hydroxyprogesterone, and 11‑ketotestosterone for congenital adrenal hyperplasia monitoringDe Baets et al., Talanta (paywalled) · source ↗

    • blood
    • dried
    • vams
    • neoteryx-mitra
    • validation
    • venous-agreement
    • pediatric
    • hormones
    • haematocrit
  2. 2025

    In children with SLE, VAMS finger-prick capillary blood gave haematocrit-adjusted MPA and MPAG concentrations indistinguishable from plasma, and the AUC from VAMS derived plasma-equivalent concentrations matched plasma AUC with R2 0.97. This supports accurate pharmacokinetically guided dosing of MMF using only three timed capillary microsamples.

    Volumetric Absorptive Microsampling Combined with Mass Spectrometry to Support Pharmacokinetically-Guided Precision Dosing of Mycophenolate Mofetil in Pediatric Lupus Nephritis PatientsZhao et al., The journal of applied laboratory medicine (paywalled) · source ↗

    • vams
    • venous-agreement
    • blood
    • tdm
    • haematocrit
    • dried
    • capillary
    • pediatric
    • immunosuppressants
    • validation
  3. 2024

    The study validated a VAMS-based LC-MS/MS method for measuring sirolimus levels, showing it was clinically equivalent to the standard whole blood method and suitable for therapeutic drug monitoring in paediatric transplant patients, supporting the use of decentralised microsampling for this purpose.

    Personalization of pharmacotherapy with sirolimus based on volumetric absorptive microsampling (VAMS) in pediatric renal transplant recipients-from LC-MS/MS method validation to clinical applicationKocur et al., Pharmacological reports : PR · source ↗

    • vams
    • venous-agreement
    • neoteryx-mitra
    • blood
    • tdm
    • haematocrit
    • dried
    • capillary
    • pediatric
    • immunosuppressants
    • validation
  4. 2023

    A study of 50 paediatric renal transplant recipients found that a validated VAMS LC-MS/MS method for tacrolimus showed high correlation with the reference method over 0.5–60 ng/mL, with minimal haematocrit effect.

    Therapeutic Drug Monitoring of Tacrolimus Based on VAMS in Renal Transplant Pediatric Recipients — LC-MS/MS Method, Hematocrit Effect, and Clinical ApplicationKocur et al., Pharmaceutics · source ↗

    • vams
    • venous-agreement
    • neoteryx-mitra
    • blood
    • tdm
    • haematocrit
    • dried
    • capillary
    • pediatric
    • immunosuppressants
    • validation
  5. 2023

    A study of 50 paediatric renal transplant recipients found that a UHPLC-MS/MS method successfully quantified mycophenolic acid in VAMS microsampling samples over a 0.10-15 µg/mL range. However, converting microsampling results to plasma equivalents using haematocrit-based regression is required for clinical interpretation, supporting patient-centric decentralised monitoring.

    Therapeutic drug monitoring of mycophenolic acid (MPA) using volumetric absorptive microsampling (VAMS) in pediatric renal transplant recipients: ultra-high-performance liquid chromatography-tandem mass spectrometry analytical method development, cross-validation, and clinical applicationKocur et al., Pharmacological reports : PR · source ↗

    • vams
    • venous-agreement
    • blood
    • tdm
    • haematocrit
    • dried
    • capillary
    • pediatric
    • immunosuppressants
    • validation