Utilizing first void urine for high-risk HPV testing for cervical cancer screening in HIV-positive women in Katete, Zambia

Urine-based HPV testing demonstrated 74% sensitivity and 52% specificity for detecting CIN2+, with performance improving when first-void collection and PCR-based assays were used. Although less sensitive than clinician-collected cervical samples, urine testing was preferred by over 90% of women, indicating potential as a complementary screening strategy to improve uptake.

Diagnostic Accuracy of Urine-Based HPV Testing Compared With Clinician-Collected Cervical Samples for Cervical Intraepithelial Neoplasia Grade 2 or Worse (CIN2+) Detection: A Systematic Review and Meta-Analysis — G et al., Cureus · source ↗

• urine
• first-void
• self-collection
• validation
• acceptability
• hpv
• sti

A time-resolved fluorescence assay detected HPV16-specific IgG in first-void urine with strong correlation to paired serum across four immunoassays in 225 healthy women, supporting first-void urine as a non-invasive alternative to venous blood for HPV antibody monitoring.

Time-resolved fluorescence (TRF) for total IgG and HPV16-specific antibody detection in first-void urine and serum: A comparative study — Lipovac et al., Journal of virological methods · source ↗

• urine
• first-void
• validation
• venous-agreement
• serology
• hpv

First-void urine IgG remained stable for at least 14 days at room temperature when stored with conservation medium. Amicon filtration with Melon Gel purification enriched HPV16-specific IgG that correlated strongly with serum levels, supporting non-invasive disease monitoring and vaccine impact assessment.

Stability, enrichment, and quantification of total and HPV16-specific IgG present in first-void urine — Téblick et al., Scientific reports · source ↗

• urine
• first-void
• validation
• serology
• hpv

First-void urine testing detected HPV with 87% sensitivity and 89% specificity, and participants reported feeling comfortable with this self-collection method. This approach could improve uptake of cervical cancer screening in decentralised settings.

The Impact of Urine-Sample HPV Testing on the Effectiveness of Screening for Cervical Cancer: An Umbrella Review — Miazga et al., Cancers · source ↗

• urine
• first-void
• self-collection
• validation
• acceptability
• hpv

A systematic review of 70 studies found that 94.7% of accuracy studies reported comparable sensitivity and specificity between self-sampling and clinician collection for HPV detection, with patient acceptability ranging from 84.2% to 100% for devices including Colli-Pee. This evidence endorses decentralised cervical screening to improve uptake.

Comparison of diagnostic accuracy and acceptability of self-sampling devices for human Papillomavirus detection: A systematic review — Aimagambetova et al., Preventive medicine reports · source ↗

• urine
• first-void
• colli-pee
• self-collection
• validation
• acceptability
• hpv