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OpenSampling

2024 · Cancers · open access

The Impact of Urine-Sample HPV Testing on the Effectiveness of Screening for Cervical Cancer: An Umbrella Review

Miazga et al.

The finding, in our words

First-void urine testing detected HPV with 87% sensitivity and 89% specificity, and participants reported feeling comfortable with this self-collection method. This approach could improve uptake of cervical cancer screening in decentralised settings.

A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.

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  1. 2025

    Urine-based HPV testing demonstrated 74% sensitivity and 52% specificity for detecting CIN2+, with performance improving when first-void collection and PCR-based assays were used. Although less sensitive than clinician-collected cervical samples, urine testing was preferred by over 90% of women, indicating potential as a complementary screening strategy to improve uptake.

    Diagnostic Accuracy of Urine-Based HPV Testing Compared With Clinician-Collected Cervical Samples for Cervical Intraepithelial Neoplasia Grade 2 or Worse (CIN2+) Detection: A Systematic Review and Meta-AnalysisG et al., Cureus · source ↗

    • urine
    • first-void
    • self-collection
    • validation
    • acceptability
    • hpv
    • sti
  2. 2024

    A systematic review of 70 studies found that 94.7% of accuracy studies reported comparable sensitivity and specificity between self-sampling and clinician collection for HPV detection, with patient acceptability ranging from 84.2% to 100% for devices including Colli-Pee. This evidence endorses decentralised cervical screening to improve uptake.

    Comparison of diagnostic accuracy and acceptability of self-sampling devices for human Papillomavirus detection: A systematic reviewAimagambetova et al., Preventive medicine reports · source ↗

    • urine
    • first-void
    • colli-pee
    • self-collection
    • validation
    • acceptability
    • hpv
  3. 2020

    First-void urine self-collection for HPV testing showed good agreement with cervical samples using the COBAS assay (kappa 0.66) but only moderate agreement with CLART (kappa 0.47). The COBAS assay achieved 63.9% sensitivity and 96.5% specificity with far fewer invalid results than CLART (1.8% versus 26.9%), and 42% of women preferred urine sampling, indicating that urine-based HPV testing is an acceptable decentralised option but requires improved analytical performance before implementation for non-participants.

    Urine collection in cervical cancer screening - analytical comparison of two HPV DNA assaysTranberg et al., BMC infectious diseases · source ↗

    • urine
    • first-void
    • self-collection
    • validation
    • acceptability
    • hpv
    • sti
  4. 2026

    This meta-analysis found that HPV testing using self-collected vaginal and urine samples showed sensitivities of 88.6% and 81% for detecting cervical precancer. Using a standardised device for first-void urine increased sensitivity to 86.6%, supporting decentralised patient-centric screening, though data on specificity and specific commercial devices were not consistently reported.

    Diagnostic Performance of HPV Testing Using Self-Collected Urine and Vaginal Samples for Detecting Cervical Precancer or Worse: A Meta-AnalysisHsiao et al., The journal of obstetrics and gynaecology research (paywalled) · source ↗

    • urine
    • first-void
    • self-collection
    • validation
    • hpv
    • sti
  5. 2026

    A study of 2500 women found 99.0% agreement between first-void urine and vaginal self-sampling for HPV detection using Cobas4800, with 98.1% of participants preferring urine. Although conducted in a resource-limited setting which may limit generalisability, these results support urine as a viable sample type for decentralised, patient-centric screening.

    Comparative efficacy, feasibility and acceptability of HPV DNA testing on first-void urine versus self-collected vaginal samples: a real-world study in a resource-limited settingBose et al., BMJ global health · source ↗

    • urine
    • first-void
    • self-collection
    • acceptability
    • hpv
    • sti