2014 · Archives of gynecology and obstetrics · paywalled
Interest of human papillomavirus DNA quantification and genotyping in paired cervical and urine samples to detect cervical lesions
Ducancelle et al.
The finding, in our words
In 230 women, HPV detection in self-collected urine showed 90 per cent agreement with cervical sampling (κ=0.80), with 97 per cent concordance for high-risk types. High viral load in urine correlated with cytological abnormalities, suggesting urine self-sampling could enable decentralised cervical cancer screening with good accuracy.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
This study found strong agreement between self-collected urine samples tested with the INDICAID HPV assay and clinician-collected swabs tested with the BD Onclarity assay for detecting high-risk HPV. The results suggest that urine-based self-collection offers a reliable alternative for cervical cancer screening, potentially improving access for women who face barriers to standard care.
A study of 100 Brazilian women with high-grade cervical lesions found excellent concordance for high-risk HPV detection between clinician-collected samples and patient-centric, self-collected vaginal or first-void urine samples. Self-sampling was preferred for comfort, though the study's single-centre setting may limit generalisability.
A smartphone based colourimetric assay that detects a metabolic byproduct of HPV infected cells in urine achieved sensitivity and specificity comparable to nucleic acid detection, offering a low cost, user friendly option for home self screening of HPV.
The hrHPV ReadyMix qPCR Kit detected high-risk HPV DNA in self-collected urine with 80.9% sensitivity and 100% specificity when compared to cervical swabs. Urine testing identified a similar HPV prevalence (6.28%) to cervical swabs (6.62%), suggesting this non-invasive method could expand cervical cancer screening programmes in low-resource settings.
Self-collected urine samples matched clinician-collected cervical samples in detecting all seven CIN3 lesions among hrHPV-positive women when extended HPV typing was used, showing 82% concordance for hrHPV detection. This indicates urine self-collection could serve as a patient-centric, decentralised screening option for cervical precancer without compromising diagnostic yield.