Follow-up of humoral immune response after HPV vaccination using first-void urine: A longitudinal cohort study

A time-resolved fluorescence assay detected HPV16-specific IgG in first-void urine with strong correlation to paired serum across four immunoassays in 225 healthy women, supporting first-void urine as a non-invasive alternative to venous blood for HPV antibody monitoring.

Time-resolved fluorescence (TRF) for total IgG and HPV16-specific antibody detection in first-void urine and serum: A comparative study — Lipovac et al., Journal of virological methods · source ↗

• urine
• first-void
• validation
• venous-agreement
• serology
• hpv

Urine sampling with the Colli-Pee device revealed a substantial burden of high-risk HPV infection among unvaccinated Thai schoolgirls, with prevalence increasing from 8.6% in grade 10 to 12.4% in grade 12, demonstrating the feasibility of decentralised screening to guide immunisation policy.

Prevalence of HPV infection among Thai schoolgirls in the north-eastern provinces in 2018: implications for HPV immunization policy — Vongpunsawad et al., IJID regions · source ↗

• urine
• first-void
• colli-pee
• serology
• hpv

This study demonstrates for the first time that HPV vaccine antibodies can be detected in first-void urine from young women, with levels correlating strongly to serum (Spearman r 0.64–0.88). This non-invasive approach could support large-scale epidemiological studies and vaccine monitoring, reducing the need for venous blood draws, though further optimisation and validation is needed.

First-void urine as a non-invasive liquid biopsy source to detect vaccine-induced human papillomavirus antibodies originating from cervicovaginal secretions — Van Keer et al., Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (paywalled) · source ↗

• venous-agreement
• first-void
• serology
• hpv
• liquid-biopsy
• urine
• validation

DNA methylation markers in first-void urine effectively detect high-grade cervical lesions in HPV-positive women, with results similar to clinician-collected samples in those over 30. This enables reliable cervical cancer screening using self-collected urine, reducing the need for invasive follow-up procedures in decentralised settings.

Evaluating DNA methylation markers and extended HPV genotyping in first-void urine for detecting high-grade cervical lesions in HPV-positive women: a cross-sectional study — Tranberg et al., BMC medicine · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• urine
• biomarkers
• genotyping

Urinary HPV testing using first-void urine collected with the Colli-Pee device showed substantial agreement with clinician-collected cervico-vaginal sampling and near-equivalent negative predictive value, supporting its use as a clinically credible non-invasive entry point for cervical screening in settings where low participation rather than analytical performance is the principal barrier.

Agreement and Diagnostic Performance of Urinary HPV Testing Versus Clinician-Collected Cervico-Vaginal Sampling: A Prospective Cross-Sectional Study in a Romanian Cohort — Nati et al., Medical sciences (paywalled) · source ↗

• urine
• first-void
• colli-pee
• hpv
• sti