Development and validation of method for analysis of favipiravir and remdesivir in volumetric absorptive microsampling with ultra high-performance liquid chromatography-tandem mass spectrophotometry

The study developed and validated a rapid LC-MS/MS method for quantifying cefazolin in capillary whole blood collected via VAMS, plasma, and plasma ultrafiltrate, showing robust performance across matrices and successful application in a pediatric pilot cohort for therapeutic drug monitoring.

Rapid, robust LC-MS/MS quantification of cefazolin in whole blood microsamples, plasma, and plasma ultrafiltrate: Analytical method validation and preliminary clinical application in pediatric population — Kocur et al., Clinica chimica acta; international journal of clinical chemistry (paywalled) · source ↗

• vams
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• antimicrobials
• capillary
• pediatric
• validation

A UPLC-MS/MS method for linezolid and metabolites in oral fluid and VAMS capillary blood satisfied ICH M10 validation requirements. Linezolid concentrations correlated strongly with venous plasma in both matrices, offering a non‑invasive option for therapeutic drug monitoring, whereas metabolites were systematically underestimated in VAMS and seldom detectable in oral fluid, restricting metabolite measurement.

Simultaneous quantification of linezolid and its metabolites (PNU-142300 and PNU-142586) in oral fluid and capillary blood by UPLC-MS/MS: method validation and clinical application using non-invasive sampling techniques — González-Berdullas et al., Journal of translational medicine (paywalled) · source ↗

• blood
• saliva
• dried
• capillary
• vams
• validation
• venous-agreement
• tdm
• antimicrobials
• haematocrit

A systematic review found mixed economic evidence for microsampling in therapeutic drug monitoring; one study reported no cost reduction with DBS home-sampling, €688 versus €676, whilst another predicted savings up to 61%. The authors caution that more work is required to assess costs alongside clinical outcomes and optimise logistics for decentralised implementation.

The economic evidence for microsampling to support therapeutic drug monitoring: A systematic review — Carland et al., British Journal of Clinical Pharmacology (paywalled) · source ↗

• vams
• blood
• economics
• dbs
• tdm
• self-collection
• dried
• antimicrobials
• pediatric
• immunosuppressants
• validation

This study validated a method for quantifying fluconazole using the Mitra device, demonstrating sufficient accuracy and precision for therapeutic drug monitoring in paediatric patients.

Determination of Fluconazole in Children in Small Blood Volumes Using Volumetric Absorptive Microsampling (VAMS) and Isocratic High-Performance Liquid Chromatography-Ultraviolet (HPLC-UV) Detection — Zimbelmann et al., Pharmaceutics · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• pediatric
• tdm
• antimicrobials

The study compared quantitative dried blood spot (qDBS) and volumetric absorptive microsampling (VAMS) devices for measuring ganciclovir levels in capillary blood from pediatric renal transplant recipients. This comparison provides evidence for choosing between microsampling devices in therapeutic drug monitoring, supporting decentralised sampling approaches that reduce patient burden while maintaining analytical accuracy.

The comparison of two volumetric microsampling devices (qDBS and VAMS) for determining ganciclovir levels in capillary blood using the LC-MS/MS technique in pediatric renal transplant recipients — Kocur et al., European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (paywalled) · source ↗

• vams
• volumetric
• blood
• qdbs
• tdm
• dried
• antimicrobials
• capillary
• pediatric
• validation