Comparison of Human Papillomavirus Genotype Detection in Paired Urine and Self-Collected Cervical Swabs: A Pilot Study

A study of 100 Brazilian women with high-grade cervical lesions found excellent concordance for high-risk HPV detection between clinician-collected samples and patient-centric, self-collected vaginal or first-void urine samples. Self-sampling was preferred for comfort, though the study's single-centre setting may limit generalisability.

Self-sampling for HPV genotyping: a study of vaginal and urine collection in Brazilian women with high-grade lesions — Castanheira et al., Clinics · source ↗

• acceptability
• first-void
• sti
• self-collection
• hpv
• urine
• genotyping

The hrHPV ReadyMix qPCR Kit detected high-risk HPV DNA in self-collected urine with 80.9% sensitivity and 100% specificity when compared to cervical swabs. Urine testing identified a similar HPV prevalence (6.28%) to cervical swabs (6.62%), suggesting this non-invasive method could expand cervical cancer screening programmes in low-resource settings.

Urinary detection of high-risk HPV DNA to enhance cervical cancer screening in developing countries — Intan et al., Microbiology spectrum · source ↗

• urine
• self-collection
• validation
• genotyping
• hpv
• sti

Self-collected urine samples detected high-risk HPV DNA in 25.5 per cent of women and 8 per cent of men by qPCR, while oral gargle samples detected HPV in 7 per cent of women and 5 per cent of men. With good diagnostic accuracy against Pap smear, qPCR on self-collected specimens offers a viable decentralised approach to HPV screening in remote populations.

Evaluating different samples & techniques for hr-HPV DNA genotyping to improve the efficiency of risk profiling for oral & cervical cancers in Sikkim, India — Gupta et al., The Indian journal of medical research · source ↗

• saliva
• urine
• self-collection
• validation
• genotyping
• hpv
• sti

Urine self-collection detected CIN2/3 with 90.2% sensitivity compared with 93.4% for cervical samples, showing moderate concordance (kappa 0.42) and similar HPV16 detection rates. This validates urine-based HPV screening as a less invasive, patient-centric approach that could expand cervical cancer screening coverage in decentralised healthcare settings.

Detection and genotyping of human papillomavirus virus (HPV): a comparative analysis of clinical performance in cervical and urine samples in Chilean women — Buchegger et al., International journal of clinical and experimental pathology (paywalled) · source ↗

• urine
• self-collection
• genotyping
• hpv
• sti

In 230 women, HPV detection in self-collected urine showed 90 per cent agreement with cervical sampling (κ=0.80), with 97 per cent concordance for high-risk types. High viral load in urine correlated with cytological abnormalities, suggesting urine self-sampling could enable decentralised cervical cancer screening with good accuracy.

Interest of human papillomavirus DNA quantification and genotyping in paired cervical and urine samples to detect cervical lesions — Ducancelle et al., Archives of gynecology and obstetrics (paywalled) · source ↗

• sti
• self-collection
• hpv
• liquid
• urine
• genotyping