Urinary detection of high-risk HPV DNA to enhance cervical cancer screening in developing countries
Intan et al.
The finding, in our words
The hrHPV ReadyMix qPCR Kit detected high-risk HPV DNA in self-collected urine with 80.9% sensitivity and 100% specificity when compared to cervical swabs. Urine testing identified a similar HPV prevalence (6.28%) to cervical swabs (6.62%), suggesting this non-invasive method could expand cervical cancer screening programmes in low-resource settings.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
Self-collected urine samples detected high-risk HPV DNA in 25.5 per cent of women and 8 per cent of men by qPCR, while oral gargle samples detected HPV in 7 per cent of women and 5 per cent of men. With good diagnostic accuracy against Pap smear, qPCR on self-collected specimens offers a viable decentralised approach to HPV screening in remote populations.
Gupta et al., The Indian journal of medical research · source ↗
This meta-analysis found that HPV testing using self-collected vaginal and urine samples showed sensitivities of 88.6% and 81% for detecting cervical precancer. Using a standardised device for first-void urine increased sensitivity to 86.6%, supporting decentralised patient-centric screening, though data on specificity and specific commercial devices were not consistently reported.
Hsiao et al., The journal of obstetrics and gynaecology research (paywalled) · source ↗
This meta-analysis found that self-collected urine samples for HPV testing had lower sensitivity for detecting high-grade lesions compared to physician-collected cervical specimens. The use of a first-void collection device such as Colli-Pee was associated with marginally higher sensitivity than general urine sampling.
Chen et al., Journal of lower genital tract disease (paywalled) · source ↗
Vaginal self-sampling achieved higher pooled sensitivity and specificity than urine self-sampling for detecting high-risk HPV, making it the preferred option, while urine self-sampling remains a useful non-invasive choice for under-screened populations.
For decentralised testing, a study found dry vaginal self-samples using Copan FLOQSwabs stored up to two weeks at room temperature maintain DNA quality. However, first-void urine collected with the Colli-Pee device may be less sensitive for HPV detection, showing lower positivity and higher cycle threshold values, median Ct 30 versus 27 for other samples.
Chu et al., BJOG : an international journal of obstetrics and gynaecology · source ↗