Stability of acetylsalicylic acid in human blood collected using volumetric absorptive microsampling (VAMS) under various drying conditions

In 55 patients, four approaches to convert dried capillary blood concentrations to plasma equivalents were evaluated. All methods except haematocrit-based conversion yielded acceptable analytical and clinical agreement for paracetamol and metabolites, supporting the reliability of capillary microsampling for decentralised therapeutic drug monitoring.

Comparative Evaluation of Approaches to Convert Microsampled Capillary Blood Concentrations to Plasma Concentrations: Paracetamol and Metabolites as a Case Study — Boffel et al., The AAPS journal (paywalled) · source ↗

• vams
• dct
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• capillary
• validation

The authors developed and validated a workflow that couples Mitra volumetric absorptive microsampling with hybridisation LC-MS/MS to quantify the antisense oligonucleotide fomivirsen in human blood. Quantitative recovery was achieved irrespective of haematocrit level or sample age, and the method demonstrated sensitivity, linearity, precision, accuracy, and four-month stability, supporting its use for therapeutic drug monitoring of biologic candidates in decentralised trials.

Volumetric absorptive microsampling coupled with hybridization LC-MS/MS for quantitation of antisense oligonucleotides — Chen et al., Bioanalysis (paywalled) · source ↗

• vams
• dct
• biologics
• neoteryx-mitra
• blood
• tdm
• haematocrit
• dried
• validation

The study found that blood concentrations of padsevonil measured using Mitra devices can reliably predict plasma concentrations with less than 9% mean bias, supporting the use of VAMS as a patient-centric alternative to venous sampling in decentralised trials. This validation strengthens confidence in microsampling for therapeutic drug monitoring where venous draws are impractical.

Clinical Bridging Studies and Modeling Approach for Implementation of a Patient Centric Sampling Technique in Padsevonil Clinical Development — Kramer et al., The AAPS journal (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• venous-agreement
• tdm
• dct

The study validated a sensitive LC-MS/MS method to measure rosuvastatin in 10 µL of blood collected using VAMS, with acceptable accuracy and precision across multiple days. Rosuvastatin remained stable in VAMS samples for 10 days at room temperature without pH buffer, supporting its use in decentralised clinical trials and patient-centric therapeutic drug monitoring.

Validation of LC-MS/MS method for determination of rosuvastatin concentration in human blood collected by volumetric absorptive microsampling (VAMS) — Moon et al., Translational and clinical pharmacology · source ↗

• vams
• dct
• neoteryx-mitra
• blood
• tdm
• dried
• validation

The study validated a VAMS-based bioanalytical method using the Tasso OnDemand device for the drug gefapixant and demonstrated a mathematical bridging relationship with standard plasma assays, supporting its use in decentralised clinical trials.

Clinical application of volumetric absorptive microsampling to the gefapixant development program — Roadcap et al., Bioanalysis (paywalled) · source ↗

• vams
• dct
• venous-agreement
• neoteryx-mitra
• blood
• tdm
• dried
• validation