Recurrent cervical cancer detection using DNA methylation markers in self-collected samples from home

The study found that testing for ASCL1/LHX8 DNA methylation in at-home collected first-void urine can detect most cervical cancers and high-grade precancers, supporting a fully molecular screening pathway without a clinical visit.

Clinical performance of ASCL1/LHX8 DNA methylation on first-void urine to screen for cervical cancer — Van Keer et al., Communications medicine · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• liquid-biopsy
• urine
• biomarkers

This literature review found that DNA methylation markers on self-collected urine or cervicovaginal swabs detected cervical intraepithelial neoplasia as well as cytology. The combination could improve screening uptake and reduce loss to follow-up, particularly in low- and middle-income countries, though more evidence is needed to identify the most efficacious methylation tests.

The current state of DNA methylation biomarkers in self-collected liquid biopsies for the early detection of cervical cancer: a literature review — Sumiec et al., Infectious agents and cancer · source ↗

• urine
• self-collection
• validation
• hpv
• sti
• biomarkers
• liquid-biopsy

In first-void urine collected with Colli-Pee, the extraction method influenced HPV and human DNA yield more than the collected volume did: standardising processing matters at least as much as standardising volume.

Impact of Collection Volume and DNA Extraction Method on the Detection of Biomarkers and HPV DNA in First-Void Urine — Téblick et al., Molecules · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• liquid-biopsy
• liquid
• urine
• biomarkers

The review concludes that first-void urine is a feasible source for molecular biomarkers, such as host and viral gene methylation, to triage high-risk HPV infections, offering a non-invasive and preferred method for women that may improve screening adherence.

First-void urine: A potential biomarker source for triage of high-risk human papillomavirus infected women — Van Keer et al., European journal of obstetrics, gynecology, and reproductive biology (paywalled) · source ↗

• urine
• first-void
• self-collection
• validation
• hpv
• biomarkers
• liquid-biopsy

DNA methylation markers in first-void urine effectively detect high-grade cervical lesions in HPV-positive women, with results similar to clinician-collected samples in those over 30. This enables reliable cervical cancer screening using self-collected urine, reducing the need for invasive follow-up procedures in decentralised settings.

Evaluating DNA methylation markers and extended HPV genotyping in first-void urine for detecting high-grade cervical lesions in HPV-positive women: a cross-sectional study — Tranberg et al., BMC medicine · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• urine
• biomarkers
• genotyping