2014 · European J. Clinical Microbiology & Infectious Diseases · paywalled
Optimization of HPV DNA detection in urine by improving collection, storage, and extraction
Vorsters et al.
The finding, in our words
Collecting a first-void sample, not midstream, and combining it immediately with a DNA-conservation buffer markedly improves urinary HPV DNA detection: suboptimal collection otherwise underestimates true prevalence.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
Self-collected vaginal and urine specimens demonstrated good to substantial agreement with clinician-collected cervical samples for detecting high-risk HPV and other sexually transmitted infections, supporting their use in decentralised screening programmes. The higher STI positivity rate among hrHPV-positive women suggests coinfections may influence cervical disease, warranting further investigation.
Giubbi et al., International journal of molecular sciences · source ↗
An independent VALHUDES evaluation found HPV screening on Colli-Pee first-void urine detected high-grade cervical lesions with sensitivity comparable to clinician-collected cervical samples, reinforcing urine self-sampling for cervical screening.
Adding polyethylene glycol to first-void urine before centrifugation markedly improved cell-free DNA recovery in the pellet, whereas low-speed centrifugation alone removed cellular DNA while keeping cell-free DNA in the supernatant. Pseudovirions pelleted reliably under all conditions, but the study revealed substantial variation between self-collected samples, highlighting the need for standardised protocols in decentralised HPV screening programmes.
Téblick et al., European journal of medical research · source ↗
Self-collected vaginal and first-void urine samples achieved substantial agreement with clinician-collected cervical samples for HPV detection using the BD Onclarity™ assay, with vaginal samples showing 95.3% overall positive agreement (kappa 0.890) and urine samples 90.6% agreement (kappa 0.776) when no clinical cut-off was applied. This supports decentralised, patient-centric cervical screening programmes.
High-risk HPV testing on first-void urine self-collected with Colli-Pee was about as sensitive, relative sensitivity 0.95, and specific for CIN2+ as clinician-taken cervical samples, supporting standardised first-void urine as a valid screening matrix.
Van Keer et al., Gynecologic Oncology (paywalled) · source ↗