2014 · European J. Clinical Microbiology & Infectious Diseases · paywalled
Optimization of HPV DNA detection in urine by improving collection, storage, and extraction
Vorsters et al.
The finding, in our words
Collecting a first-void sample, not midstream, and combining it immediately with a DNA-conservation buffer markedly improves urinary HPV DNA detection: suboptimal collection otherwise underestimates true prevalence.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
An independent VALHUDES evaluation found HPV screening on Colli-Pee first-void urine detected high-grade cervical lesions with sensitivity comparable to clinician-collected cervical samples, reinforcing urine self-sampling for cervical screening.
Adding polyethylene glycol to first-void urine before centrifugation markedly improved cell-free DNA recovery in the pellet, whereas low-speed centrifugation alone removed cellular DNA while keeping cell-free DNA in the supernatant. Pseudovirions pelleted reliably under all conditions, but the study revealed substantial variation between self-collected samples, highlighting the need for standardised protocols in decentralised HPV screening programmes.
High-risk HPV testing on first-void urine self-collected with Colli-Pee was about as sensitive, relative sensitivity 0.95, and specific for CIN2+ as clinician-taken cervical samples, supporting standardised first-void urine as a valid screening matrix.
In women with HPV, first-void urine collected with the Colli-Pee device had higher HPV DNA concentrations than urine collected with a standard cup, although this advantage disappeared at high HPV DNA loads. Timing of collection (morning versus later) did not affect HPV DNA copies.
This meta-analysis found that HPV testing using self-collected vaginal and urine samples showed sensitivities of 88.6% and 81% for detecting cervical precancer. Using a standardised device for first-void urine increased sensitivity to 86.6%, supporting decentralised patient-centric screening, though data on specificity and specific commercial devices were not consistently reported.