2023 · International journal of molecular sciences · open access
New Perspectives of Multiplex Mass Spectrometry Blood Protein Quantification on Microsamples in Biological Monitoring of Elderly Patients
Vialaret et al.
The finding, in our words
The analysis quantified 62 proteins with satisfactory analytical performance, and 48 proteins showed significant correlation between 2 µL plasma microsamples and dried blood spots, supporting the use of dried blood microsampling for home collection and predictive medicine in elderly patients.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
The study demonstrated that qPCR-based epigenetic immune cell counting from capillary dried blood spots agreed with venous blood and conventional flow cytometry in healthy donors, and then identified lymphopenia, neutrophilia and a lowered lymphocyte-to-neutrophil ratio in 103 COVID-19 patients versus 113 healthy controls, with naive B cell frequency tracking disease severity, suggesting that filter-paper blood collection could enable remote immune monitoring in home-isolated patients.
The analysis indicates that dried blood spots and volumetric absorptive microsampling enable minimally invasive monitoring of prohibited substances, with volumetric absorptive microsampling offering improved quantitative reliability over traditional dried spots.
Volumetric absorptive microsampling showed better precision than dried blood spots and a metabolic profile closer to whole blood, with stable signalling lipids for 24 hours at room temperature but significant changes after one week unless stabilised, indicating feasibility for decentralised sampling with the need for storage strategies.
Capillary dried blood spots, after haematocrit-dependent conversion, showed good agreement with plasma for 25-hydroxyvitamin D quantification, with 90 per cent of results within 20 per cent of plasma and substantial to almost perfect agreement in status classification, supporting reliable home self-collection for large-scale vitamin D monitoring.
This study validated a dried blood spot method for tacrolimus monitoring and demonstrated agreement with venous whole blood measurements in kidney transplant recipients. It also found that dried blood spot analysis of the biomarker CXCL-10 was elevated in patients experiencing rejection or infection, supporting its use for decentralised monitoring.