2023 · Journal of visualized experiments : JoVE · paywalled
Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
Ngo et al.
The finding, in our words
The study describes a protocol for measuring the low-abundance cancer biomarker progastrin-releasing peptide in dried serum spots and volumetric absorptive microsampling devices, finding that VAMS yielded higher signal intensities than dried serum spots.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
Researchers successfully validated an analytical method for measuring creatinine across plasma and volumetric absorptive microsampling devices, demonstrating strong correlation between conventional plasma and dried microsamples. This provides a reliable, patient-centric approach for monitoring kidney function remotely during transplant follow-up.
Kocur et al., European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (paywalled) · source ↗
An optimised VAMS workflow for blood proteomics increased protein identifications 4-fold in plasma and 2.1-fold in whole blood compared with liquid processing, with mean CVs below 11%, and whole blood showed greater robustness and storage stability at room temperature for up to 14 days. This enables reliable patient-centric microsampling for longitudinal biomarker studies.
Karsten et al., Journal of proteome research (paywalled) · source ↗
Untargeted metabolomic profiles from three blood microsampling devices aligned more closely with whole blood than with plasma, and all devices distinguished sex based on amino acids, lipids, and acylcarnitines. This validates that device choice can be tailored to the metabolites of interest for decentralised human biomonitoring.
Avella et al., Metabolomics : Official journal of the Metabolomic Society · source ↗
A streamlined workflow quantified up to 10,000 protein groups and post-translational modifications from 20 microlitres of dried blood collected by volumetric absorptive microsampling, with mass spectrometry acquisition under two hours. The study established stability profiles and best practices for dried blood proteomics, supporting its feasibility for decentralised precision medicine and disease phenotyping.
Foster et al., Journal of proteome research (paywalled) · source ↗
This study validated a method for measuring the alcohol biomarker phosphatidylethanol using two volumetric absorptive microsampling devices, finding that the Mitra device met all validation criteria and agreed with venous sampling. The Capitainer device was deemed suitable but showed reduced accuracy at higher concentrations, with a small proportional negative bias compared to venous results.
Andreassen et al., Journal of analytical toxicology (paywalled) · source ↗