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OpenSampling

2020 · PloS one · open access

High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay

McDade et al.

The finding, in our words

An ELISA detecting SARS-CoV-2 IgG antibodies from self-collected dried blood spots identified seropositivity in 80 per cent of household contacts of confirmed cases, 36 per cent of community participants without known infection, and 97 per cent of convalescent individuals. This finger-prick capillary sampling method offers a decentralised alternative to venepuncture for seroprevalence studies.

A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.

Read the source ↗DOI 10.1371/journal.pone.0237833PubMed 32797108

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Papers that share these labels

  1. 2026

    The study validated a semi-quantitative protocol for Epstein-Barr virus serology using dried blood spots, demonstrating 98.8% sensitivity and 96.5% specificity compared to paired venous serum samples. The successful validation in a self-sampling cohort confirms the suitability of this patient-centric microsampling approach for large-scale seroprevalence studies and decentralised trials.

    A dried blood spot protocol for high-throughput semi-quantitative analysis of Epstein-Barr Virus VCA IgG and EBNA IgG serologies based on the Roche Elecsys system — Rubio-Acero et al., Virology journal · source ↗

    • blood
    • dried
    • capillary
    • dbs
    • self-collection
    • validation
    • venous-agreement
    • serology

  2. 2023

    Dried blood spot eluates showed high agreement with serum for SARS-CoV-2 IgG against Spike and Nucleocapsid (r≈0.96; qualitative φ≈0.98–1.0) and remained stable after 6–8 hours of storage, supporting use of capillary DBS for serosurveys and decentralised testing.

    Dried blood spot eluates are suitable for testing of SARS-CoV-2 IgG antibodies targeting Spike protein 1 and Nucleocapsid protein — Guttmann et al., Biochemistry and biophysics reports · source ↗

    • venous-agreement
    • blood
    • dbs
    • self-collection
    • dried
    • serology
    • capillary
    • validation

  3. 2022

    Dried blood spot sampling achieved high sensitivity and specificity for SARS-CoV-2 antibody detection with strong correlation to paired serum, enabling virtually painless, decentralised specimen collection in community and remote settings where venepuncture is difficult.

    Evaluation of serological assays for SARS-CoV-2 antibody testing from dried blood spots collected from cohorts with prior SARS-CoV-2 infection — Catlett et al., Journal of clinical virology plus · source ↗

    • blood
    • dried
    • capillary
    • dbs
    • self-collection
    • validation
    • venous-agreement
    • serology

  4. 2021

    Capillary dried blood spot samples analysed with the Roche Elecsys anti-SARS-CoV-2 assay on COBAS 6000 systems agreed with paired serum results in 98.9% of cases and remained accurate at 86.7% after 9 days, supporting their use for decentralised COVID-19 serosurveillance and large-scale screening.

    Analysis of SARS-CoV-2 antibodies from dried blood spot samples with the Roche Elecsys Immunochemistry method — Fontaine & Saez, Practical laboratory medicine · source ↗

    • blood
    • dried
    • capillary
    • dbs
    • self-collection
    • validation
    • venous-agreement
    • serology

  5. 2021

    Capillary blood self-sampling showed near-perfect agreement with venous sampling for SARS-CoV-2 IgG detection, achieving a Cohen's kappa of 0.88. Samples remained stable at room temperature for seven days, and 38 of 39 participants successfully collected adequate volumes, supporting its use for decentralised serological testing.

    Self-sampling of capillary blood for SARS-CoV-2 serology — Brown et al., Scientific reports · source ↗

    • blood
    • liquid
    • dried
    • capillary
    • dbs
    • self-collection
    • validation
    • venous-agreement
    • serology