Genetic individual identification from dried urine spots: A complementary tool to drug monitoring and anti-doping testing
Grignani et al.
The finding, in our words
Dried urine spots on paper cards can yield reliable DNA for individual identification for up to 12 weeks. Female samples produce complete genetic profiles directly, whereas male urine requires a thirty-fold concentration step, which supports decentralised anti-doping and drug monitoring by enabling genetic verification of sample authenticity.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
This review highlights that microsampling techniques such as dried blood spots and volumetric absorptive microsampling offer superior alternatives to traditional sampling for the mass spectrometry detection of prohibited substances in anti-doping analysis. It provides guidance on selecting appropriate analytical methods and sample pretreatment strategies to ensure sensitive detection and reliable storage conditions.
Maykar et al., Critical reviews in analytical chemistry (paywalled) · source ↗
A multi-targeted procedure covered 237 prohibited substances across 11 WADA classes in dried urine spots, with environmental-stability testing for refrigeration-free transport.
Mazzarino et al., Analytical and Bioanalytical Chemistry (paywalled) · source ↗
The authors found that a single microdose of recombinant EPO was detectable up to 72 hours using ITP and CP methods, though the Tasso microsampling device showed lower sensitivity than Mitra and Capitainer. Additionally, isotope ratio mass spectrometry successfully detected testosterone micro-dosing in all analysed samples, supported by serum testosterone levels.
Heiland et al., Drug testing and analysis · source ↗
Confirms urine is the second-most-applied VAMS matrix in forensics, collecting a consistent volume regardless of viscosity and enabling room-temperature storage and shipping.
This review surveyed the use of VAMS devices for analysing endogenous metabolites and biomarkers across the full analytical workflow, finding them reliable for biological analysis with improved analyte stability over liquid blood at ambient temperature and a straightforward acquisition model well suited to decentralised diagnostics.