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OpenSampling

2024 · Immunity, inflammation and disease · open access

Evaluation of self-collected nasal, urine, and saliva samples for molecular detection of SARS-CoV-2 using an EUA approved RT-PCR assay and a laboratory developed LAMP SARS-CoV-2 test

Purcell-Wiltz et al.

The finding, in our words

Self-collected saliva samples for SARS-CoV-2 testing yielded higher viral loads than nasal or urine specimens and showed moderate agreement (kappa 0.52) with nasal swabs. A colourimetric LAMP assay achieved 90% concordance with RT-PCR, offering a rapid alternative deployable in clinics, workplaces, and homes for decentralised surveillance.

A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.

Labels

  1. 2025

    Integrative Advances in Dengue Virus Diagnostics: From Technological Innovation to Tiered Diagnostic Systems

    This review summarises how incorporating saliva and urine into home-based point-of-care systems expands diagnostic accessibility for dengue virus, aligning with the WHO REASSURED framework for affordable and rapid testing.

    Shi et al., Journal of medical virology (paywalled) · source ↗

    • saliva
    • urine
    • self-collection
    • validation
    • serology
  2. 2024

    The Emergence of Saliva as a Diagnostic and Prognostic Tool for Viral Infections

    This review found saliva comparable to blood and urine for detecting viral genetic material and antibodies, supporting patient-centric monitoring and decentralised surveillance. The authors state that standardised protocols and further validation are required for routine clinical use.

    Oliveira et al., Viruses · source ↗

    • blood
    • self-collection
    • serology
    • hpv
    • urine
    • validation
    • saliva
    • biomarkers
  3. 2023

    Toward Rapid and Accurate Molecular Diagnostics at Home

    This review summarises progress toward integrating automated sample preparation with on-chip nucleic acid amplification for home testing of self-collected blood, urine, saliva and stool specimens. It identifies that user-friendly, combined systems may enable rapid, accurate molecular diagnostics outside central laboratories, which is relevant for decentralised infectious disease screening.

    Liu & Lee, Advanced materials (paywalled) · source ↗

    • blood
    • saliva
    • urine
    • stool
    • self-collection
    • validation
    • serology
  4. 2021

    Integrated Point-of-Care Molecular Diagnostic Devices for Infectious Diseases

    This review identifies sample preparation as the key bottleneck limiting rapid home testing for infectious diseases, despite advances in nucleic acid amplification and signal transduction. It emphasises saliva, blood, urine and stool as primary self-collected specimens and discusses on-chip integration strategies to simplify operation for end-users, which could transform decentralised infectious disease diagnostics.

    Liu et al., Accounts of chemical research (paywalled) · source ↗

    • blood
    • saliva
    • urine
    • stool
    • self-collection
    • validation
    • serology
  5. 2026

    Validation of Dried Saliva for Molecular Diagnostics

    The study found that self-collected dried saliva on FishburneTabs achieved 92% accuracy for viral RNA detection compared to standard liquid saliva samples. This method supports decentralised testing by enabling reliable sample storage and transport for upper respiratory infections.

    Mora et al., ACS measurement science au · source ↗

    • self-collection
    • dried
    • serology
    • validation
    • saliva