2017 · Rapid communications in mass spectrometry : RCM · paywalled
Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies
Kyle et al.
The finding, in our words
After 15 years of room-temperature storage, dried blood spots showed substantial degradation of polar metabolites with only one remaining statistically significant, whereas 36 lipids remained stable and correlated with matched serum samples. This indicates DBS may be viable for long-term lipid studies but unreliable for polar metabolite analysis in longitudinal decentralised research.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
Volumetric absorptive microsampling showed better precision than dried blood spots and a metabolic profile closer to whole blood, with stable signalling lipids for 24 hours at room temperature but significant changes after one week unless stabilised, indicating feasibility for decentralised sampling with the need for storage strategies.
Thangavelu et al., Analytical and bioanalytical chemistry · source ↗
A comparison of three patient-centric dried blood microsampling devices, paper DBS, Mitra and Tasso-M20, found strong to excellent correlation with traditional venous plasma for measuring branched-chain amino acids and ketoacids. Participants reported high acceptability and expressed a strong willingness to use these devices for decentralised self-collection.
In an untargeted metabolomics study, microsampling devices, particularly the Mitra and Capitainer, yielded metabolic profiles comparable or superior to plasma in feature number and intensity, and in the precision and stability of some metabolites. This supports their potential for large-scale, decentralised metabolic profiling, though the captured metabolite profile was application-dependent.
An optimized 4D-lipidomics UHPLC-HRMS protocol using stable isotope internal standards enabled semi-quantitative profiling of 432 unique lipid features from 10 ̢L dried blood spot samples with high analytical reproducibility. The workflow demonstrates the viability of capillary blood microsampling for large-scale, remote population lipidomics and metabolic profiling.
Microsampling enables less invasive, patient-centric self-collection of capillary blood for remote monitoring of metabolites and lipids, overcoming conventional venipuncture constraints. Recent device innovations address dried blood spot limitations, particularly haematocrit and volume variations, expanding decentralised applications in population health, drug discovery and multi-omics research.