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OpenSampling

2023 · F&S reports · open access

At-home urine estrone-3-glucuronide quantification predicts oocyte retrieval outcomes comparably with serum estradiol

Nakhuda et al.

The finding, in our words

At-home urine oestrone-3-glucuronide measured with the Mira Fertility Tracker during gonadotropin stimulation correlated well with paired serum oestradiol (r = 0.761) and predicted mature oocyte yield to a similar degree, suggesting that patient-centric oestrogen monitoring could reduce reliance on clinic-based venous draws during fertility treatment.

A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.

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  1. 2025

    Similar accuracy and patient experience with different one-step ovulation predictor kits

    All five at-home urine ovulation predictor kits demonstrated high accuracy (92–97%) when compared with blood LH levels, though sensitivity varied widely from 38% to 77% with Clinical Guard the lowest. Patient experience was comparable across kits, though Clinical Guard was slightly less favoured for future use.

    Vanderhoff et al., Fertility and sterility (paywalled) · source ↗

    • blood
    • urine
    • self-collection
    • validation
    • venous-agreement
    • acceptability
    • fertility
    • hormones
  2. 2023

    Validation of urinary reproductive hormone measurements using a novel smartphone connected reader

    The Inito Fertility Monitor demonstrated strong agreement with laboratory ELISA for measuring urinary estrone-3-glucuronide, pregnanediol glucuronide and luteinising hormone, with coefficients of variation around 5%. It identified a novel ovulation confirmation criterion that distinguished ovulatory from anovulatory cycles with 100% specificity and an area under the curve of 0.98, supporting accurate decentralised fertility monitoring.

    Pattnaik et al., Scientific reports · source ↗

    • first-void
    • self-collection
    • fertility
    • hormones
    • urine
    • validation
  3. 2021

    Direct measurement of pregnanediol 3-glucuronide (PDG) in dried urine spots by liquid chromatography-mass spectrometry to detect ovulation

    Handelsman et al. validated a direct LCMS assay for pregnanediol glucuronide in dried urine spots without deconjugation. In ten women monitored across one menstrual cycle, using two or three consecutive first-void samples reduced false-negative ovulation detection from eight per cent to 2.6 per cent and 1.9 per cent respectively, enabling accurate home-based fertility assessment.

    Handelsman et al., The Journal of steroid biochemistry and molecular biology (paywalled) · source ↗

    • urine
    • dried
    • first-void
    • self-collection
    • validation
    • fertility
    • hormones
    • steroids
  4. 2026

    Salivary estradiol and progesterone across the menstrual cycle: Association and agreement with serum concentrations

    Self-collected salivary progesterone and the ratio of estradiol to progesterone showed strong agreement with serum levels, supporting the use of saliva as a noninvasive matrix for monitoring the menstrual cycle.

    Goldenstein et al., Physiological reports · source ↗

    • venous-agreement
    • blood
    • self-collection
    • fertility
    • hormones
    • liquid
    • saliva
  5. 2026

    From venipuncture to self-sampling dried blood spots: a shift in monitoring testosterone levels

    Home DBS sampling gave testosterone results that agreed well with venous blood in men on intramuscular testosterone undecanoate, but showed falsely high values in those using topical gel, likely from skin contamination. Patients preferred home collection for its convenience, suggesting DBS is viable for decentralised monitoring of injectable testosterone if patients are taught proper technique.

    Olthof et al., Clinical chemistry and laboratory medicine (paywalled) · source ↗

    • blood
    • dried
    • capillary
    • dbs
    • self-collection
    • validation
    • venous-agreement
    • acceptability
    • fertility
    • hormones
    • tdm