2023 · Clinical and translational allergy · open access
Application of a dried blood spot based proteomic and genetic assay for diagnosing hereditary angioedema
Iuraşcu et al.
The finding, in our words
Dried blood spot analysis combined with LC-MS proteomics and DNA sequencing reliably quantified C1-INH and C4 proteins and identified SERPING1 mutations, matching plasma-based diagnostic patterns for hereditary angioedema.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
This systematic review of 67 studies involving 34,739 kidney disease patients found dried blood microsampling was mainly used for immunosuppressant therapeutic drug monitoring and kidney function assessment. The approach offered cost savings, was preferred by patients for home self-collection, and provides a patient-centric opportunity to upscale longitudinal sampling and reduce participation bias in decentralised kidney disease research.
Lamond et al., Journal of clinical laboratory analysis · source ↗
Dried blood spots provide a minimally invasive, low-volume and cost-effective approach for measuring pollutants, genetic markers, clinical biomarkers and drug adherence in population studies, yet the haematocrit effect introduces a significant error in concentration assessment, meaning further validation is essential for decentralised diagnostic use.
McClendon-Weary et al., Current environmental health reports (paywalled) · source ↗
The analysis indicates that dried blood spots and volumetric absorptive microsampling enable minimally invasive monitoring of prohibited substances, with volumetric absorptive microsampling offering improved quantitative reliability over traditional dried spots.
Volumetric absorptive microsampling showed better precision than dried blood spots and a metabolic profile closer to whole blood, with stable signalling lipids for 24 hours at room temperature but significant changes after one week unless stabilised, indicating feasibility for decentralised sampling with the need for storage strategies.
Thangavelu et al., Analytical and bioanalytical chemistry · source ↗
Capillary dried blood spots, after haematocrit-dependent conversion, showed good agreement with plasma for 25-hydroxyvitamin D quantification, with 90 per cent of results within 20 per cent of plasma and substantial to almost perfect agreement in status classification, supporting reliable home self-collection for large-scale vitamin D monitoring.
Heughebaert et al., Clinical chemistry and laboratory medicine (paywalled) · source ↗