Screening for hypothyroidism in Down syndrome using the capillary thyroid stimulating hormone method

A liquid chromatography-tandem mass spectrometry method was successfully validated for measuring androstenedione, 17α-hydroxyprogesterone, and 11-ketotestosterone in dried blood samples collected via volumetric absorptive microsampling. The dried samples remained stable at room temperature for up to a week and after postal transit, and plasma concentrations could be reliably estimated from the microsamples when adjusting for haematocrit.

Analytical validation of a dried blood microsampling method to measure androstenedione, 17α‑hydroxyprogesterone, and 11‑ketotestosterone for congenital adrenal hyperplasia monitoring — De Baets et al., Talanta (paywalled) · source ↗

• blood
• dried
• vams
• neoteryx-mitra
• validation
• venous-agreement
• pediatric
• hormones
• haematocrit

Capillary fingerstick sampling showed high correlation and agreement with venous sampling for serum AMH but with proportional bias, so it may improve access to testing yet should not be considered fully interchangeable without further validation.

Comparison of serum anti-Müllerian hormone concentrations measured in capillary fingerstick and venous samples from Korean reproductive-aged women — Lavadia et al., Clinical and experimental reproductive medicine (paywalled) · source ↗

• blood
• capillary
• validation
• venous-agreement
• acceptability
• fertility
• hormones

Fingerstick capillary sampling showed strong agreement with venous sampling for mycophenolic acid, tacrolimus and cyclosporine A, with acceptable bias and improved agreement for mycophenolic acid after haematocrit correction. This minimally invasive approach may reduce procedural burden and support outpatient or home based therapeutic drug monitoring in children.

Minimally Invasive Therapeutic Drug Monitoring of Immunosuppressants in Children with Kidney Diseases: Validation of Fingerstick Sampling Using LC-MS/MS — Ishii et al., Pharmaceuticals · source ↗

• venous-agreement
• blood
• tdm
• haematocrit
• capillary
• pediatric
• immunosuppressants
• validation

This study validated a method requiring only 10 microlitres of serum and found high agreement between fingerstick capillary samples and venous serum for monitoring twelve antibiotics. The results suggest capillary serum sampling is a feasible, minimally invasive alternative that avoids the haematocrit effect associated with whole blood microsampling.

Feasibility verification of fingerstick capillary microsampling replacing venipuncture for therapeutic drug monitoring of 12 antibiotics using a rapid LC-MS/MS assay — Chen et al., Talanta (paywalled) · source ↗

• blood
• liquid
• capillary
• validation
• venous-agreement
• pediatric
• tdm
• antimicrobials

Home DBS sampling gave testosterone results that agreed well with venous blood in men on intramuscular testosterone undecanoate, but showed falsely high values in those using topical gel, likely from skin contamination. Patients preferred home collection for its convenience, suggesting DBS is viable for decentralised monitoring of injectable testosterone if patients are taught proper technique.

From venipuncture to self-sampling dried blood spots: a shift in monitoring testosterone levels — Olthof et al., Clinical chemistry and laboratory medicine (paywalled) · source ↗

• blood
• dried
• capillary
• dbs
• self-collection
• validation
• venous-agreement
• acceptability
• fertility
• hormones
• tdm